不同致癌化学物质诱导的实验性大鼠胶质母细胞瘤组织株的形态学和分子生物学特征

Morphological and Molecular Biological Characteristics of Experimental Rat Glioblastoma Tissue Strains Induced by Different Carcinogenic Chemicals.

作者信息

Sentyabreva Alexandra, Miroshnichenko Ekaterina, Artemova Daria, Alekseeva Anna, Kosyreva Anna

机构信息

Avtsyn Research Institute of Human Morphology of "Petrovsky National Research Centre of Surgery", 117418 Moscow, Russia.

Research Institute of Molecular and Cellular Medicine, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.

出版信息

Biomedicines. 2024 Mar 22;12(4):713. doi: 10.3390/biomedicines12040713.

DOI:10.3390/biomedicines12040713

PMID:38672069

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11048177/

Abstract

UNLABELLED

Glioblastoma (GBM) is a highly aggressive human neoplasm with poor prognosis due to its malignancy and therapy resistance. To evaluate the efficacy of antitumor therapy, cell models are used most widely, but they are not as relevant to human GBMs as tissue models of gliomas, closely corresponding to human GBMs in cell heterogeneity. In this work, we compared three different tissue strains of rat GBM 101.8 (induced by DMBA), GBM 11-9-2, and GBM 14-4-5 (induced by ENU).

MATERIALS AND METHODS

We estimated different gene expressions by qPCR-RT and conducted Western blotting and histological and morphometric analysis of three different tissue strains of rat GBM.

RESULTS

GBM 101.8 was characterized by the shortest period of tumor growth and the greatest number of necroses and mitoses; overexpression of , , , , and ; and downregulated expression of , , and ; as well as a high level of HIF-1α protein content. GBM 11-9-2 and GBM 14-4-5 were relevant to low-grade gliomas and characterized by downregulated expression; furthermore, a low content of CD133 protein was found in GBM 11-9-2.

CONCLUSIONS

GBM 101.8 is a reliable model for further investigation due to its similarity to high-grade human GBMs, while GBM 11-9-2 and GBM 14-4-5 correspond to Grade 2-3 gliomas.

摘要

未标记

胶质母细胞瘤（GBM）是一种极具侵袭性的人类肿瘤，因其恶性程度和治疗抗性而预后较差。为了评估抗肿瘤治疗的疗效，细胞模型应用最为广泛，但它们与人类GBM的相关性不如胶质瘤组织模型，胶质瘤组织模型在细胞异质性方面与人类GBM密切对应。在这项工作中，我们比较了大鼠GBM的三种不同组织菌株101.8（由二甲基苯并蒽诱导）、GBM 11 - 9 - 2和GBM 14 - 4 - 5（由乙基亚硝基脲诱导）。

材料与方法

我们通过qPCR - RT估计不同基因的表达，并对大鼠GBM的三种不同组织菌株进行蛋白质印迹以及组织学和形态计量学分析。

结果

GBM 101.8的特征在于肿瘤生长周期最短，坏死和有丝分裂数量最多；、、、和过表达；、和表达下调；以及HIF - 1α蛋白含量水平较高。GBM 11 - 9 - 2和GBM 14 - 4 - 5与低级别胶质瘤相关，其特征在于表达下调；此外，在GBM 11 - 9 - 2中发现CD133蛋白含量较低。