Neves Matheus Morais, Guerra Renan Faria, de Lima Isabela Lemos, Arrais Thomas Santos, Guevara-Vega Marco, Ferreira Flávia Batista, Rosa Rafael Borges, Vieira Mylla Spirandelli, Fonseca Belchiolina Beatriz, Sabino da Silva Robinson, Silva Murilo Vieira da
Biotechnology in Experimental Models Laboratory-LABME, Federal University of Uberlândia, Uberlândia 38405-330, MG, Brazil.
Rodents Animal Facilities Complex, Federal University of Uberlandia, Uberlândia 38400-902, MG, Brazil.
Microorganisms. 2024 Apr 20;12(4):833. doi: 10.3390/microorganisms12040833.
Currently, there is a wide application in the literature of the use of the Fourier Transform Infrared Spectroscopy (FTIR) technique. This basic tool has also proven to be efficient for detecting molecules associated with hosts and pathogens in infections, as well as other molecules present in humans and animals' biological samples. However, there is a crisis in science data reproducibility. This crisis can also be observed in data from experimental animal models (EAMs). When it comes to rodents, a major challenge is to carry out sanitary monitoring, which is currently expensive and requires a large volume of biological samples, generating ethical, legal, and psychological conflicts for professionals and researchers. We carried out a survey of data from the relevant literature on the use of this technique in different diagnostic protocols and combined the data with the aim of presenting the technique as a promising tool for use in EAM. Since FTIR can detect molecules associated with different diseases and has advantages such as the low volume of samples required, low cost, sustainability, and provides diagnostic tests with high specificity and sensitivity, we believe that the technique is highly promising for the sanitary and stress and the detection of molecules of interest of infectious or non-infectious origin.
目前，傅里叶变换红外光谱（FTIR）技术在文献中有广泛应用。这个基本工具已被证明在检测感染中与宿主和病原体相关的分子以及人类和动物生物样本中存在的其他分子方面是有效的。然而，科学数据的可重复性存在危机。这种危机在实验动物模型（EAM）的数据中也能观察到。对于啮齿动物而言，一个主要挑战是进行卫生监测，目前这成本高昂且需要大量生物样本，给专业人员和研究人员带来伦理、法律和心理冲突。我们对相关文献中关于该技术在不同诊断方案中的使用数据进行了调查，并将这些数据结合起来，目的是将该技术展示为一种有望用于实验动物模型中的工具。由于傅里叶变换红外光谱能够检测与不同疾病相关的分子，并且具有所需样本量少、成本低、可持续性等优点，还能提供具有高特异性和灵敏度的诊断测试，我们认为该技术在卫生监测、应激以及检测感染性或非感染性来源的感兴趣分子方面极具前景。
