乳清钙螯合物促进低钙饮食大鼠的钙吸收和骨骼健康。

-Derived Whey-Calcium Chelate Promotes Calcium Absorption and Bone Health of Rats Fed a Low-Calcium Diet.

作者信息

Hu Wei, Pei Zhiwen, Xia Aonan, Jiang Yang, Yang Bo, Liu Xiaoming, Zhao Jianxin, Zhang Hao, Chen Wei

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

出版信息

Nutrients. 2024 Apr 11;16(8):1127. doi: 10.3390/nu16081127.

DOI:10.3390/nu16081127

PMID:38674818

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11053418/

Abstract

This study investigated the characteristics of -derived whey-calcium chelate (LHWCC) and its effect on the calcium absorption and bone health of rats. Fourier-transform infrared spectroscopy showed that carboxyl oxygen atoms, amino nitrogen atoms, and phosphate ions were the major binding sites with calcium in LHWCC, which has a sustained release effect in simulated in vitro digestion. LHWCC had beneficial effects on serum biochemical parameters, bone biomechanics, and the morphological indexes of the bones of calcium-deficient rats when fed at a dose of 40 mg Ca/kg BW for 7 weeks. In contrast to the inorganic calcium supplement, LHWCC significantly upregulated the gene expression of transient receptor potential cation V5 (TRPV5), TRPV6, PepT1, calcium-binding protein-D9k (Calbindin-D9k), and a calcium pump (plasma membrane Ca-ATPase, PMCA1b), leading to promotion of the calcium absorption rate, whereas Ca(PO) only upregulated the TRPV6 channel in vivo. These findings illustrate the potential of LHWCC as an organic calcium supplement.

摘要

本研究调查了乳清钙螯合物（LHWCC）的特性及其对大鼠钙吸收和骨骼健康的影响。傅里叶变换红外光谱表明，羧基氧原子、氨基氮原子和磷酸根离子是LHWCC中与钙结合的主要位点，其在体外模拟消化中具有缓释作用。当以40mg Ca/kg体重的剂量喂养缺钙大鼠7周时，LHWCC对血清生化参数、骨生物力学和骨骼形态指标具有有益作用。与无机钙补充剂相比，LHWCC显著上调瞬时受体电位阳离子通道蛋白V5（TRPV5）、TRPV6、肽转运蛋白1（PepT1）、钙结合蛋白-D9k（Calbindin-D9k）和钙泵（质膜钙ATP酶，PMCA1b）的基因表达，从而促进钙吸收率，而磷酸钙（Ca(PO)）仅在体内上调TRPV6通道。这些发现说明了LHWCC作为有机钙补充剂的潜力。