转染细胞中氯霉素乙酰转移酶活性的检测：一种基于高效液相色谱的快速灵敏方法。

Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method.

作者信息

Young S L, Jackson A E, Puett D, Melner M H

出版信息

DNA. 1985 Dec;4(6):469-75. doi: 10.1089/dna.1985.4.469.

DOI:10.1089/dna.1985.4.469

Abstract

An assay based on high-performance liquid chromatography (HPLC) has been developed for determining the expression of the acetyl coenzyme A: chloramphenicol 3-O-acetyltransferase gene in transfected cells. The assay is based on the separation of extracted, nonradioactive chloramphenicol and its acetylated derivatives by reverse-phase HPLC, using UV absorbance for detection. In addition to eliminating the need for costly [14C]chloramphenicol, this assay is as sensitive and much faster than the commonly used method involving thin-layer chromatography.

摘要

已开发出一种基于高效液相色谱（HPLC）的检测方法，用于测定转染细胞中乙酰辅酶A：氯霉素3 - O - 乙酰转移酶基因的表达。该检测方法基于通过反相HPLC分离提取的非放射性氯霉素及其乙酰化衍生物，并使用紫外吸收进行检测。除了无需使用昂贵的[14C]氯霉素外，该检测方法与常用的薄层色谱法一样灵敏，且速度更快。