State Key Laboratory of Drug Research and State Key Laboratory of Chemical Biology, Carbohydrate-Based Drug Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
State Key Laboratory of Drug Research and State Key Laboratory of Chemical Biology, Carbohydrate-Based Drug Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, China.
Curr Opin Chem Biol. 2024 Jun;80:102460. doi: 10.1016/j.cbpa.2024.102460. Epub 2024 Apr 27.
Protein glycosylation is one of the most common and important post-translational modifications of proteins involved in regulating glycoprotein functions. The chemoenzymatic glycan labeling strategy allows rapid, efficient, and selective interrogation of glycoproteins. Glycoproteomics identifies protein glycosylation events at a large scale, providing information such as peptide sequences, glycan structures, and glycosylated sites. This review discusses the recent development of chemoenzymatic labeling strategies for glycoprotein analysis, mainly including glycoprotein and glycosite profiling. Furthermore, we highlight the chemoenzymatic enrichment approaches in mass spectrometry analysis for three classes of glycan modifications, including N-glycosylation, O-GlcNAcylation, and mucin-type O-glycosylation. Finally, we highlight the emerging trends in new tools and cutting-edge technologies available for glycoproteomic research.
蛋白质糖基化是涉及调节糖蛋白功能的最常见和最重要的蛋白质翻译后修饰之一。化学酶糖基化标记策略允许快速、高效和选择性地研究糖蛋白。糖蛋白质组学在大规模上鉴定蛋白质糖基化事件，提供肽序列、聚糖结构和糖基化位点等信息。本综述讨论了糖基化分析的化学酶标记策略的最新进展，主要包括糖蛋白和糖基化位点分析。此外，我们还强调了用于三种糖基化修饰（包括 N-糖基化、O-GlcNAcylation 和粘蛋白型 O-糖基化）的质谱分析中的化学酶富集方法。最后，我们强调了用于糖蛋白质组学研究的新工具和前沿技术的新兴趋势。
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