基于适体的牛奶中β-乳球蛋白的快速灵敏生物传感器。
A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk.
机构信息
School of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, China.
Hangzhou Institute of Medicine Chinese Academy of Sciences, 310022, China.
出版信息
Anal Methods. 2024 May 16;16(19):3039-3046. doi: 10.1039/d4ay00460d.
Beta-lactoglobulin (β-Lg), a prominent milk protein, is a major contributor to milk allergies. The quantitative assessment of β-Lg is a valuable method for assessing the allergenic potential of dairy products. In this study, a specific aptamer, β-Lg-01, with an affinity constant () of 28.6 nM for β-Lg was screened through seven rounds of magnetic bead SELEX (MB-SELEX). A novel bio-layer interferometry (BLI)-based aptasensor was developed, which had a limit of detection (LOD) of 0.3 ng mL, a linear range of 1.5 ng mL-15 μg mL, and a recovery rate of 102-116% among the milk samples. This aptasensor provides a potential tool for the detection and risk assessment of β-Lg within 10 min.
β-乳球蛋白（β-Lg）是一种主要的牛奶蛋白，是牛奶过敏的主要原因之一。定量评估β-Lg 是评估乳制品致敏潜能的一种有价值的方法。在这项研究中，通过七轮磁珠 SELEX（MB-SELEX）筛选出一种对β-Lg 具有亲和力常数（Kd）为 28.6 nM 的特异性适配体β-Lg-01。开发了一种基于新型生物层干涉法（BLI）的适配体传感器，该传感器在牛奶样品中的检测限（LOD）为 0.3 ng mL，线性范围为 1.5 ng mL-15 μg mL，回收率为 102-116%。该适配体传感器为在 10 分钟内检测和评估β-Lg 提供了一种潜在的工具。
Zotero 插件