角膜塑形术的污染与卫生合规性的关联。
Association Between Contamination and Hygiene Compliance in Orthokeratology.
机构信息
Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.
Department of Ophthalmology, Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C.
出版信息
In Vivo. 2024 May-Jun;38(3):1229-1235. doi: 10.21873/invivo.13559.
BACKGROUND/AIM: Given the characteristics of Serratia marcescens (S. marcescens), this study aimed at investigating its presence in the hands and contact lens cases of orthokeratology wearers, along with the status of bacterial contamination.
PATIENTS AND METHODS
The 39 patients received the questionnaires about the background of orthokeratology and hygiene habits. A total of 39 contact lens cases and 39 hand samples from the patients were collected at Show Chwan Memorial Hospital from June to August in 2020 and sent to National Chung Cheng University for DNA extraction and PCR identification.
RESULTS
The results indicated a detection rate of 5.13% for S. marcescens in the contact lens cases and 12.82% in the hand samples. Additionally, 66.67% of contact lens case samples and 30.77% of hand samples found positive for 16s bacterial amplicons. The relationship between hand contamination and the duration of contact lens usage were revealed for both S. marcescens (p=0.021) and 16s bacterial amplicons (p=0.048).
CONCLUSION
The results indicated that hand hygiene is more critical than focusing on contact lens hygiene when it comes to preventing S. marcescens infections. Nevertheless, both proper hand and contact lens hygiene practices can reduce the detection of bacterial eye pathogens, especially a common intestinal bacterium.
背景/目的：鉴于黏质沙雷氏菌（Serratia marcescens，S. marcescens）的特点，本研究旨在调查其在角膜塑形术佩戴者的手部和接触镜盒中的存在情况及其细菌污染状况。
患者和方法
39 名患者接受了关于角膜塑形术和卫生习惯背景的问卷调查。2020 年 6 月至 8 月，在秀传纪念医院共采集了 39 个接触镜盒和 39 个患者手部样本，并送往国立中正大学进行 DNA 提取和 PCR 鉴定。
结果
接触镜盒中 S. marcescens 的检出率为 5.13%，手部样本中为 12.82%。此外，66.67%的接触镜盒样本和 30.77%的手部样本的 16s 细菌扩增子呈阳性。手部污染与接触镜使用时间的关系对于 S. marcescens（p=0.021）和 16s 细菌扩增子（p=0.048）均有显示。
结论
结果表明，预防黏质沙雷氏菌感染时，手部卫生比关注接触镜卫生更为重要。然而，适当的手部和接触镜卫生习惯都可以减少眼部细菌病原体的检出，尤其是常见的肠道细菌。
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