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三维支架中海藻酸钠-明胶共培养的人脐带间充质干细胞条件培养液促进毛发生长。

Promotion of hair growth by a conditioned medium from human umbilical cord mesenchymal stem cells cultivated in a 3D scaffold of gelatin sponge.

机构信息

Department of Plastic Surgery and Burns Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.

Research Center for Translational Medicine, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.

出版信息

Eur J Med Res. 2024 May 4;29(1):270. doi: 10.1186/s40001-024-01830-7.

DOI:10.1186/s40001-024-01830-7
PMID:38704575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11069168/
Abstract

BACKGROUND

This study aims to investigate the effects of a conditioned medium (CM) from human umbilical cord mesenchymal stem cells (HuMSCs) cultivated in gelatin sponge (GS-HuMSCs-CM) on hair growth in a mouse model.

METHODS

CM was collected from the HuMSCs cultivated in a monolayer or in a gelatin sponge. Vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), keratinocyte growth factor (KGF), and hepatocyte growth factor (HGF) levels in CMs were measured by enzyme-linked immunosorbent assays (ELISAs). A hair loss model by a C57 BL/6J mouse was prepared. The effects of GS-HuMSCs-CM and HuMSCs on hair regrowth in mice were investigated by intradermal injection in the depilated back skin with normal saline (NS) as the control. The time for hair regrowth and full covering in depilated areas was observed, and the hair growth was evaluated histologically and by grossly measuring hair length and diameter.

RESULTS

Compared with monolayer cultured cells, the three-dimensional (3D) culture of HuMSCs in gelatin sponge drastically increased VEGF, IGF-1, KGF, and HGF production. GS-HuMSCs-CM and HuMSCs injection both promoted hair regeneration in mice, while GS-HuMSCs-CM presented more enhanced effects in hair length, hair diameter, and growth rate. GS-HuMSCs-CM significantly promoted angiogenesis in injected skin areas, which might also contribute to faster hair regrowth.

CONCLUSION

GS-HuMSCs-CM exerted significant effects on inducing hair growth and promoted skin angiogenesis in C57BL/6J mice.

摘要

背景

本研究旨在探讨在明胶海绵中培养的人脐带间充质干细胞（HuMSCs）的条件培养基（CM）对小鼠模型中毛发再生的影响。

方法

从单层培养或明胶海绵中培养的 HuMSCs 中收集 CM。通过酶联免疫吸附试验（ELISA）测量 CM 中的血管内皮生长因子（VEGF）、胰岛素样生长因子-1（IGF-1）、角质细胞生长因子（KGF）和肝细胞生长因子（HGF）水平。通过 C57BL/6J 小鼠制备脱发模型。通过皮内注射将 NS 作为对照注入脱毛背部皮肤，研究 GS-HuMSCs-CM 和 HuMSCs 对小鼠毛发再生的影响。观察毛发再生和脱毛区域完全覆盖的时间，并通过组织学和大体测量毛发长度和直径评估毛发生长。

结果

与单层培养细胞相比，明胶海绵中 HuMSCs 的三维（3D）培养大大增加了 VEGF、IGF-1、KGF 和 HGF 的产生。GS-HuMSCs-CM 和 HuMSCs 注射均促进了小鼠的毛发再生，而 GS-HuMSCs-CM 在毛发长度、直径和生长速度方面表现出更显著的效果。GS-HuMSCs-CM 显著促进了注射皮肤区域的血管生成，这也可能有助于更快的毛发再生。

结论

GS-HuMSCs-CM 对诱导毛发生长有显著作用，并促进 C57BL/6J 小鼠皮肤的血管生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/4a81e5bda189/40001_2024_1830_Fig10_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/4a81e5bda189/40001_2024_1830_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/0bfcedef05b8/40001_2024_1830_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/93af95466189/40001_2024_1830_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/57526a90bc8a/40001_2024_1830_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/cf0b87779c7a/40001_2024_1830_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/9705c6ccd707/40001_2024_1830_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/f0ba57ae1985/40001_2024_1830_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/6d137e53a42b/40001_2024_1830_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/5426037f0c50/40001_2024_1830_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/8b152a4f674c/40001_2024_1830_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/11069168/4a81e5bda189/40001_2024_1830_Fig10_HTML.jpg

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