An antifouling epoxy coated metal surface containing silica-immobilized carbonic anhydrase supraparticles for CO capture through microalgae.

Carbonic anhydrase (CA) has a promising application as a green and efficient biocatalyst for CO capture, and many successful cases of immobilizing CA have been reported. However, CA antifouling coatings on metal for CO sequestration have rarely been reported. Herein, dimeric CA from Sulfurihydrogenibium azorense (SazCA) with a ferritin tag, which was prepared by low-speed centrifugation with high yield, was adopted as a free enzyme and encapsulated in the sol-gel silica. The silica-immobilized CAs were dispersed into the commercialized metal-antifouling epoxy resin paint to obtain CA coated nickel foams, which had excellent stability, with 90 % and 67 % residual activity after 28 days of incubation at 30 °C and 60 °C, respectively. The CA coated nickel foams remained 60 % original activity after 6 cycles of use within 28 days. Then, a CA-microalgae carbon capture device was constructed using the CA coated nickel foams and Chlorella. The growth rate of Chlorella was significantly increased and the biomass of Chlorella increased by 29 % compared with control after 7 days of incubation. Due to the simple and cost-effective preparation process, sustainable and efficient CO absorption, this easy-to-scale up CA coated nickel foam has great potential in CA assisted microalgae-based CO capture and carbon neutrality.