Occurrence of bone fractures and parathyroid hyperplasia in paraphysectomized frogs (Rana catesbeiana).

The physiological role of the amphibian paraphysis was studied by removing the paraphysis from bullfrog tadpoles (Rana catesbeiana) and observing change in frog weight, bone development, and parathyroid glands 22 months later. Development was similar in control and paraphysectomized frogs until 6 months when the experimental frogs began to show greater weight gain which persisted for the remaining 16 months. At the time of sacrifice (22 months after paraphysectomy), the parathyroid glands of the experimental frogs were enlarged with a tenfold increase in wet weight and a sevenfold increase in dry weight. The glands from experimental frogs had numerous cysts filled with eosinophilic staining material; histologically the cell nuclei were rounded and the cytoplasm was vesiculated. At the time of sacrifice the experimental frogs had knoblike deformities along the shafts of the long bones. These deformities were due to callus formation around spontaneous bone fractures as revealed by X-rays of the tibia-fibulas and femurs. The dry weight was greater but calcium content less in femurs from experimental frogs. These findings indicate the paraphysis plays a role in calcium metabolism, possibly by producing a factor necessary for calcium action on cells.