Braley-Mullen H, Johnson M, Sharp G C, Kyriakos M
Cell Immunol. 1985 Jun;93(1):132-43. doi: 10.1016/0008-8749(85)90394-6.
Spleen cells from CBA/J or SJL mice sensitized with mouse thyroglobulin (MTg) and lipopolysaccharide (LPS) could be activated in vitro with MTg to transfer experimental autoimmune thyroiditis (EAT) to normal syngeneic recipients. EAT induced by these transferred cells was similar in incidence and severity to EAT induced by active immunization of mice with MTg and adjuvant and cells from EAT-resistant Balb/c mice could not be activated to induce EAT. The specific antigen MTg was required both for initial sensitization of the mice and for activation of spleen cells in vitro. The cells that were active in transferring EAT to mice were shown to be T cells. Removal of B cells from the cultured spleen cells had no effect on the ability of the cells to induce EAT.
用小鼠甲状腺球蛋白（MTg）和脂多糖（LPS）致敏的CBA/J或SJL小鼠的脾细胞，可在体外被MTg激活，从而将实验性自身免疫性甲状腺炎（EAT）转移给正常的同基因受体。这些转移细胞诱导的EAT在发病率和严重程度上与用MTg和佐剂对小鼠进行主动免疫诱导的EAT相似，而来自抗EAT的Balb/c小鼠的细胞不能被激活以诱导EAT。特异性抗原MTg对于小鼠的初始致敏和体外脾细胞的激活都是必需的。将EAT转移给小鼠的活性细胞显示为T细胞。从培养的脾细胞中去除B细胞对细胞诱导EAT的能力没有影响。
