Zhang Wei, Dai Miao, Zhu Ye, Li Siyuan, Sun Ying, Liu Xiaoya, Li Xiaojie
Key laboratory of synthetic and biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Lihu Street 1800, Wuxi, 214122, China.
Bioact Mater. 2024 May 6;37:549-562. doi: 10.1016/j.bioactmat.2024.03.037. eCollection 2024 Jul.
Zinc (Zn) alloys have demonstrated significant potential in healing critical-sized bone defects. However, the clinical application of Zn alloys implants is still hindered by challenges including excessive release of zinc ions (Zn), particularly in the early stage of implantation, and absence of bio-functions related to complex bone repair processes. Herein, a biodegradable aliphatic polycarbonate drug-eluting coating was fabricated on zinc-lithium (Zn-Li) alloys to inhibit Zn release and enhance the osteogenesis, angiogenesis, and bacteriostasis of Zn alloys. Specifically, the photo-curable aliphatic polycarbonates were co-assembled with simvastatin and deposited onto Zn alloys to produce a drug-loaded coating, which was crosslinked by subsequent UV light irradiation. During the 60 days long-term immersion test, the coating showed distinguished stable drug release and Zn release inhibition properties. Benefiting from the regulated release of Zn and simvastatin, the coating facilitated the adhesion, proliferation, and differentiation of MC3T3-E1 cells, as well as the migration and tube formation of EA.hy926 cells. Astonishingly, the coating also showed remarkable antibacterial properties against both and . The rabbit critical-size femur bone defects model demonstrated that the drug-eluting coating could efficiently promote new bone formation and the expression of platelet endothelial cell adhesion molecule-1 (CD31) and osteocalcin (OCN). The enhancement of osteogenesis, angiogenesis, and bacteriostasis is achieved by precisely controlling of the released Zn at an appropriate level, as well as the stable release profile of simvastatin. This tailored aliphatic polycarbonate drug-eluting coating provides significant potential for clinical applications of Zn alloys implants.
锌(Zn)合金在修复临界尺寸骨缺损方面已显示出巨大潜力。然而,锌合金植入物的临床应用仍面临诸多挑战,包括锌离子(Zn)过度释放,尤其是在植入早期,以及缺乏与复杂骨修复过程相关的生物功能。在此,在锌锂(Zn-Li)合金上制备了一种可生物降解的脂肪族聚碳酸酯药物洗脱涂层,以抑制锌的释放,并增强锌合金的成骨、血管生成和抑菌作用。具体而言,将光固化脂肪族聚碳酸酯与辛伐他汀共组装并沉积在锌合金上,以制备载药涂层,随后通过紫外线照射使其交联。在为期60天的长期浸泡试验中,该涂层表现出卓越的稳定药物释放和锌释放抑制性能。得益于锌和辛伐他汀的调控释放,该涂层促进了MC3T3-E1细胞的黏附、增殖和分化,以及EA.hy926细胞的迁移和管形成。令人惊讶的是,该涂层对[具体细菌名称1]和[具体细菌名称2]均表现出显著的抗菌性能。兔临界尺寸股骨骨缺损模型表明,药物洗脱涂层可有效促进新骨形成以及血小板内皮细胞黏附分子-1(CD31)和骨钙素(OCN)的表达。通过将释放的锌精确控制在适当水平以及辛伐他汀的稳定释放曲线,实现了成骨、血管生成和抑菌作用的增强。这种定制的脂肪族聚碳酸酯药物洗脱涂层为锌合金植入物的临床应用提供了巨大潜力。