Maastricht Multimodal Molecular Imaging (M4i) Institute, Maastricht University, Maastricht, the Netherlands.
Maastricht Multimodal Molecular Imaging (M4i) Institute, Maastricht University, Maastricht, the Netherlands.
Drug Discov Today. 2024 Jul;29(7):104027. doi: 10.1016/j.drudis.2024.104027. Epub 2024 May 17.
Various analytical technologies have been developed for the study of target-ligand interactions. The combination of these technologies gives pivotal information on the binding mechanism, kinetics, affinity, residence time, and changes in molecular structures. Mass spectrometry (MS) offers structural information, enabling the identification and quantification of target-ligand interactions. Surface plasmon resonance (SPR) provides kinetic information on target-ligand interaction in real time. The coupling of MS and SPR complements each other in the studies of target-ligand interactions. Over the last two decades, the capabilities and added values of SPR-MS have been reported. This review summarizes and highlights the benefits, applications, and potential for further research of the SPR-MS approach.
已经开发出各种分析技术来研究靶标-配体相互作用。这些技术的结合提供了关于结合机制、动力学、亲和力、停留时间和分子结构变化的关键信息。质谱 (MS) 提供结构信息，能够识别和定量靶标-配体相互作用。表面等离子体共振 (SPR) 实时提供靶标-配体相互作用的动力学信息。MS 和 SPR 的结合在靶标-配体相互作用的研究中相互补充。在过去的二十年中，已经报道了 SPR-MS 的功能和附加值。本综述总结和强调了 SPR-MS 方法的优势、应用和进一步研究的潜力。
