Department of Traumatology and Orthopedics of Zaporizhzhia State Medical and Pharmaceutical University, Ukraine.
Department of Pathological Anatomy and Forensic Medicine of Zaporizhzhia State Medical and Pharmaceutical University, Ukraine.
J ISAKOS. 2024 Aug;9(4):615-619. doi: 10.1016/j.jisako.2024.05.011. Epub 2024 May 18.
The use of infrapatellar fat pad adipose stem cells (IPFP-ASCs) shows an age-independent proliferation and differentiation potential. In addition, the pronounced chondrogenic potential of IPFP-ASCs makes them promising candidates for research for use in other methods of regenerative therapy. The purpose of this study was to ascertain the presence and compare the relative abundance of cells exhibiting an immunohistochemical profile characteristic of adipose-derived mesenchymal stem cells in selected samples of the stromal vascular fraction (SVF) obtained from the IPFP and subcutaneous fat tissue.
A direct immunohistochemical study was carried out in serial paraffin sections of the SVF of the infrapatellar fat pad (IPFP) and subcutaneous tissue, using monoclonal antibodies. The minimum criteria were established by the International Society for Cell Therapy to ensure the identity of mesenchymal stem cells use CD73, CD90, and CD105 as positive markers and CD34, CD31, and CD45 as a negative.
According to the results of histological, immunohistochemical, morphometric, and statistical studies, it was found that in the SVF of IPFP and subcutaneous adipose tissue, the relative number of cells with the profile CD105+, CD73+, CD34+, CD31-, CD45- in the standard field of view (×200), the SVF of IPFP was 1.58%, whereas the SVF of subcutaneous adipose tissue was 6.92 %, which was statistically significantly greater by 4.38 times (p < 0.05).
The presence of a sufficient number of mesenchymal stromal cells in IPFP in combination with their topographic relationship with the structures of the joint determines the use of the SVF of the IPFP for the treatment of diseases of the knee joint.
III.
髌下脂肪垫脂肪干细胞（IPFP-ASCs）的使用具有与年龄无关的增殖和分化潜能。此外，IPFP-ASCs 明显的软骨形成潜力使它们成为其他再生治疗方法研究的有前途的候选者。本研究的目的是确定在从髌下脂肪垫（IPFP）和皮下组织获得的基质血管部分（SVF）的选定样本中存在并比较表现出脂肪来源间充质干细胞免疫组织化学特征的细胞的相对丰度。
使用单克隆抗体对髌下脂肪垫（IPFP）和皮下组织的 SVF 的连续石蜡切片进行直接免疫组织化学研究。国际细胞治疗学会建立了最低标准，以确保间充质干细胞的身份使用 CD73、CD90 和 CD105 作为阳性标志物，CD34、CD31 和 CD45 作为阴性标志物。
根据组织学、免疫组织化学、形态计量学和统计学研究的结果，发现 IPFP 和皮下脂肪组织的 SVF 中，标准视野（×200）中具有 CD105+、CD73+、CD34+、CD31-、CD45- 特征的细胞的相对数量，IPFP 的 SVF 为 1.58%，而皮下脂肪组织的 SVF 为 6.92%，统计学上显著高 4.38 倍（p<0.05）。
IPFP 中存在足够数量的间充质基质细胞，加上它们与关节结构的拓扑关系，决定了使用 IPFP 的 SVF 治疗膝关节疾病。
III。
