Liu Guanqi, Yan Ziyu, Guo Yuzhu, Guo Chuanbin, Tan Chengwen, Zhu Jianhua, Han Jianmin
Central laboratory, Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing Key Laboratory of Digital Stomatology, NHC Key Laboratory of Digital Stomatology, NMPA Key Laboratory for Dental Materials, Beijing, 100081, China.
School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, China.
Heliyon. 2024 Apr 25;10(9):e30286. doi: 10.1016/j.heliyon.2024.e30286. eCollection 2024 May 15.
In this work, the corrosion behavior of pure Mg, Mg3Ag, Mg6Ag, and MgZnYNd alloys in different fixatives (ethyl alcohol (EA), 85 % ethyl alcohol (85 % EA), 10 % neutral buffered formalin (10 % NBF), 4 % glutaric dialdehyde (4 % GD), and 4 % paraformaldehyde (4 % PFA)) was investigated to provide a valuable reference for the selection of fixatives during the histological evaluation of Mg implants. Through the hydrogen evolution test, pH test, and corrosion morphology and product characterization, it was found that corrosion proceeded slowest in the EA and 85 % EA groups, slightly faster in 4 % GD, faster in 10 % NBF, and fastest in 4 % PFA. After corrosion, the EA group surface remained unchanged, while the 85%EA group surface developed minor cracks and warping. The 4%GD fixative formed a dense needle-like protective layer on the Mg substrate. The 10%NBF group initially grew a uniform layer, but later developed irregular pits due to accelerated corrosion. In contrast, the 4%PFA solution caused more severe corrosion attributed to chloride ions. The main corrosion products in the EA and 85%EA groups were MgO and Mg(OH), while the other fixatives containing diverse ions also yielded phosphates like Mg(PO) and MgHPO. In 4 % PFA, AgCl formed on the surface of Mg6Ag alloy after corrosion. Therefore, to minimize Mg alloy corrosion without compromising staining quality, EA or 85 % EA is recommended, while 4 % PFA is not recommended due to its significant impact.
在本研究中，研究了纯镁、Mg3Ag、Mg6Ag和MgZnYNd合金在不同固定剂（乙醇（EA）、85%乙醇（85%EA）、10%中性缓冲福尔马林（10%NBF）、4%戊二醛（4%GD）和4%多聚甲醛（4%PFA））中的腐蚀行为，为镁植入物组织学评估过程中固定剂的选择提供有价值的参考。通过析氢试验、pH测试以及腐蚀形态和产物表征发现，EA和85%EA组的腐蚀进行得最慢，4%GD组稍快，10%NBF组更快，4%PFA组最快。腐蚀后，EA组表面保持不变，而85%EA组表面出现轻微裂纹和翘曲。4%GD固定剂在镁基体上形成致密的针状保护层。10%NBF组最初生长出均匀的层，但后来由于腐蚀加速而出现不规则凹坑。相比之下，4%PFA溶液由于氯离子导致更严重的腐蚀。EA和85%EA组的主要腐蚀产物是MgO和Mg(OH)，而其他含有不同离子的固定剂还产生了磷酸盐，如Mg(PO)和MgHPO。在4%PFA中，Mg6Ag合金腐蚀后表面形成AgCl。因此，为了在不影响染色质量的情况下使镁合金腐蚀最小化，建议使用EA或85%EA，而由于其显著影响不建议使用4%PFA。
