Han Mengqing, Wang Chunhao, Zhu Wenhui, Pan Yuemin, Huang Lili, Nie Jiajun
Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, China.
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Hortic Res. 2024 Mar 14;11(5):uhae078. doi: 10.1093/hr/uhae078. eCollection 2024 May.
is emerging as a devastating pathogenic fungus causing anthracnose in a wide range of horticultural crops, particularly fruits. Exploitation of nonhost resistance (NHR) represents a robust strategy for plant disease management. Perception of core effectors from phytopathogens frequently leads to hypersensitive cell death and resistance in nonhost plants; however, such core effectors in and their signaling components in non-hosts remain elusive. Here, we found a virulent strain isolated from pear exhibits non-adaptation in the model plant . Perception of secreted molecules from appears to be a dominant factor in NHR, and four novel core effectors-CfCE4, CfCE25, CfCE61, and CfCE66-detected by were, accordingly, identified. These core effectors exhibit cell death-inducing activity in and accumulate in the apoplast. With a series of CRISPR/Cas9-edited mutants or gene-silenced plants, we found the coreceptor BAK1 and helper NLRs including ADR1, NRG1, and NRCs mediate perceptions of these core effectors in . Concurrently, multiple genes encoding cell surface immune receptors and intracellular immune receptors were greatly induced by . This work represents the first characterization of the repertoire of core effectors responsible for NHR. Significantly, the novel core effectors and their signaling components unveiled in this study offered insights into a continuum of layered immunity during NHR and will be helpful for anthracnose disease management in diverse horticultural crops.
正成为一种具有毁灭性的致病真菌，在多种园艺作物尤其是水果上引发炭疽病。利用非寄主抗性（NHR）是植物病害管理的一种有效策略。感知来自植物病原体的核心效应子常常会导致非寄主植物发生超敏细胞死亡和产生抗性；然而，在[具体真菌名称]中的此类核心效应子及其在非寄主中的信号传导成分仍不清楚。在这里，我们发现从梨中分离出的一种致病力强的[具体真菌名称]菌株在模式植物[具体植物名称]中表现出不适应性。感知来自[具体真菌名称]的分泌分子似乎是NHR中的一个主导因素，因此，通过[具体方法]鉴定出了四个新的核心效应子——CfCE4、CfCE25、CfCE61和CfCE66。这些核心效应子在[具体植物名称]中表现出诱导细胞死亡的活性，并在质外体中积累。通过一系列CRISPR/Cas9编辑的突变体或基因沉默植物，我们发现共受体BAK1和辅助NLRs（包括ADR1、NRG1和NRCs）介导了[具体植物名称]对这些核心效应子的感知。同时，多个编码细胞表面免疫受体和细胞内免疫受体的[具体植物名称]基因被[具体真菌名称]强烈诱导。这项工作首次对负责NHR的[具体真菌名称]核心效应子库进行了表征。重要的是，本研究中揭示的新核心效应子及其信号传导成分，为NHR过程中连续的分层免疫提供了见解，并将有助于多种园艺作物的炭疽病病害管理。
