通过合成核糖开关在人工细胞内实现可切换和正交的基因表达控制。
Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches.
机构信息
Earth-Life Science Institute, Tokyo Institute of Technology, Ookayama 2-12-1, Meguro-Ku, Tokyo 152-8550, Japan.
School of Life Science and Technology, Tokyo Institute of Technology, Ookayama 2-12-1, Meguro-Ku, Tokyo 152-8550, Japan.
出版信息
Chem Commun (Camb). 2024 Jun 4;60(46):5972-5975. doi: 10.1039/d4cc00965g.
Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well as DNA-templated encoding reporter genes regulated by these orthogonal riboswitches inside liposomes, and achieved switchable and orthogonal control over gene expression by external stimulation with the cognate ligands.
在这里，我们报告了两个新的合成核糖体开关，它们可以响应 ASP2905 和茶碱，并在体外无细胞蛋白合成 (CFPS) 系统中发挥作用。我们将 CFPS 系统以及由这些正交核糖体开关调控的 DNA 模板编码报告基因包封在脂质体中，并通过外源性配体的刺激实现了对基因表达的可切换和正交控制。
Zotero 插件