The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Zhengzhou Stomatological Hospital, Zhengzhou, China.
PLoS One. 2024 May 21;19(5):e0303147. doi: 10.1371/journal.pone.0303147. eCollection 2024.
To show the effect of the phosphate group on the remineralization process of early enamel caries mediated by amelogenin peptide.
Freshly extracted, completed, and crack-free bovine teeth were used to create artificial early enamel caries, which were randomly divided into four groups: Group A: fluorination remineralized solution treatment group; Group B: pure remineralized solution treatment group. Group C: 100 g/ml recombinant Amelogenin peptide remineralized solution treatment group (with single phosphate group on N-terminus); Group D: 100 g/ml non-phosphorylated recombinant Amelogenin peptide remineralized solution treatment group (without single phosphate group on N-terminus). For 12 days, fresh remineralized solutions were replaced daily. Transverse microradiography (TMR) was used after remineralization to determine mineral loss and demineralization depth before and after each sample's remineralization. Each sample's depth of remineralization and mineral acquisition were then determined.
The recombinant amelogenin peptide group significantly outperformed the non-phosphorylated amelogenin peptide group in terms of mineral acquisition and mineralization depth (P<0.05).
The recombinant Amelogenin's solitary phosphate group at the N-terminus helps recombinant Amelogenin to encourage the remineralization process of early enamel caries.
展示磷酸基团对釉原蛋白肽介导的早期釉质龋再矿化过程的影响。
使用新鲜、完整、无裂缝的牛牙制作人工早期釉质龋，随机分为四组：A 组：氟化再矿化溶液处理组；B 组：纯再矿化溶液处理组。C 组：100g/ml 重组釉原蛋白肽再矿化溶液处理组（N 端有单个磷酸基团）；D 组：100g/ml 非磷酸化重组釉原蛋白肽再矿化溶液处理组（N 端无单个磷酸基团）。每天更换新鲜的再矿化溶液，共 12 天。再矿化后采用横向显微放射摄影术（TMR），测定每个样本再矿化前后的脱矿深度和矿物损失。然后确定每个样本的再矿化深度和矿物质获取量。
重组釉原蛋白肽组在矿物质获取和矿化深度方面明显优于非磷酸化釉原蛋白肽组（P<0.05）。
重组釉原蛋白 N 端的单个磷酸基团有助于重组釉原蛋白促进早期釉质龋的再矿化过程。
