组织学正常的胆囊结石中存在的免疫组织化学炎症。

Division of Translational Medical Sciences, Nottingham Digestive Diseases Centre, School of Medicine, University of Nottingham, Queen's Medical Centre, Nottingham, UK.

National Institute for Health Research Nottingham Biomedical Research Centre, Nottingham University Hospitals and University of Nottingham, Queen's Medical Centre, Nottingham, UK.

World J Surg. 2024 Jul;48(7):1662-1673. doi: 10.1002/wjs.12219. Epub 2024 May 22.

BACKGROUND

The aim of this study was to establish features of inflammation in histologically normal gallbladders with gallstones and compare the expression of inflammatory markers in acutely and chronically inflamed gallbladders.

METHODS

Immunohistochemistry was performed on formalin-fixed paraffin-embedded gallbladders for tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-2R, and substance p in three groups: Group I (n = 60) chronic cholecystitis, Group II (n = 57) acute cholecystitis and Group III (n = 45) histologically normal gallbladders with gallstones. Expression was quantified using the H-scoring system.

RESULTS

Median, interquartile range expression of mucosal IL-2R in Groups I (2.65, 0.87-7.97) and II (12.30, 6.15-25.55) was significantly increased compared with group III (0.40, 0.10-1.35, p < 0.05). Submucosal IL-2R expression in Groups I (2.0, 1.12-4.95) and II (10.0, 5.95-14.30) was also significantly increased compared with Group III (0.50, 0.15-1.05, p < 0.05). There was no difference in the lymphoid cell IL-6 expression between Groups I (5.95, 1.60-18.15), II (6.10, 1.1-36.15) and III (8.30, 2.60-26.35, p > 0.05). Epithelial IL-6 expression of Group III (8.3, 2.6-26.3) was significantly increased compared with group I (0.5, 0-10.2, p < 0.05) as was epithelial TNF-α expression in Group III (85.0, 70.50-92.0) compared with Groups I (72.50, 45.25.0-85.50, p < 0.05) and II (61.0, 30.0-92.0, p < 0.05). Lymphoid cell Substance P expression in Groups I (1.90, 1.32-2.65) and II (5.62, 2.50-20.8) was significantly increased compared with Group III (1.0,1.0-1.30, p < 0.05). Epithelial cell expression of Substance P in Group III (121.7, 94.6-167.8) was significantly increased compared with Groups I (75.7, 50.6-105.3, p < 0.05) and II (78.9, 43.5-118.5, p < 0.05).

CONCLUSION

Histologically normal gallbladders with gallstones exhibited features of inflammation on immunohistochemistry.

背景

本研究旨在确定伴有胆结石的组织学正常胆囊中的炎症特征，并比较急、慢性胆囊炎中炎症标志物的表达。

方法

对三组胆囊组织（I 组，n=60，慢性胆囊炎；II 组，n=57，急性胆囊炎；III 组，n=45，组织学正常伴胆结石的胆囊）的福尔马林固定石蜡包埋胆囊进行肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-2R 和 P 物质的免疫组织化学染色。采用 H 评分系统对表达进行定量。

结果

与 III 组相比，I 组（2.65，0.87-7.97）和 II 组（12.30，6.15-25.55）的黏膜 IL-2R 中位值、四分位间距表达明显升高（p<0.05）。I 组（2.0，1.12-4.95）和 II 组（10.0，5.95-14.30）的黏膜下 IL-2R 表达也明显升高（p<0.05）。I 组（5.95，1.60-18.15）、II 组（6.10，1.1-36.15）和 III 组（8.30，2.60-26.35）的淋巴细胞 IL-6 表达无差异（p>0.05）。III 组上皮细胞 IL-6 表达（8.3，2.6-26.3）明显高于 I 组（0.5，0-10.2，p<0.05），III 组上皮细胞 TNF-α 表达（85.0，70.50-92.0）明显高于 I 组（72.50，45.25.0-85.50，p<0.05）和 II 组（61.0，30.0-92.0，p<0.05）。I 组（1.90，1.32-2.65）和 II 组（5.62，2.50-20.8）的淋巴细胞 P 物质表达明显升高（p<0.05）。与 I 组（75.7，50.6-105.3）和 II 组（78.9，43.5-118.5）相比，III 组上皮细胞 P 物质表达（121.7，94.6-167.8）明显升高（p<0.05）。