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超声辅助提取和纳滤膜浓缩沙巴菰(Plinia peruviana (Poir.) Govaerts)果皮中的酚类化合物。

Ultrasound-assisted extraction and concentration of phenolic compounds from jabuticaba sabará (Plinia peruviana (Poir.) Govaerts) peel by nanofiltration membrane.

机构信息

Food Engineering Department, URI Erechim, Av. Sete de Setembro 1621, 99709-910 Erechim, RS, Brazil.

Food Engineering Department, URI Erechim, Av. Sete de Setembro 1621, 99709-910 Erechim, RS, Brazil.

出版信息

Food Chem. 2024 Sep 30;453:139690. doi: 10.1016/j.foodchem.2024.139690. Epub 2024 May 17.

Abstract

Jabuticaba peel, rich in antioxidants, offering health benefits. In this study, the extraction of phenolic compounds from jabuticaba peel using ultrasound-assisted (UA) and their subsequent concentration by nanofiltration (NF) employing a polyamide 200 Da membrane was evaluated. The UA extractions were conducted using the Central Composite Rotatable Design (CCRD) 22 methodology, with independent variables extraction time (11.55 to 138 min) and temperature (16.87 to 53.3 °C), and fixed variables mass to ethanol solution concentration at pH 1.0 (1:25 g/mL), granulometry (1 mm), and ultrasonic power (52.8 W). The maximum concentrations obtained were 700.94 mg CE/100 g for anthocyanins, 945.21 mg QE/100 g for flavonoids, 133.19 mg GAE/g for phenols, and an antioxidant activity IC50 of 24.36 μg/mL. Key phenolic compounds identified included cyanidin-3-glucoside, delphinidin-3-glucoside, and various acids like syringic and gallic. NF successfully concentrated these compounds, enhancing their yield by up to 45%. UA and NF integrate for sustainable extraction.

摘要

巴西莓果皮富含抗氧化剂,有益健康。本研究采用超声辅助提取(UA)法从巴西莓果皮中提取酚类化合物,并用截留分子量为 200 Da 的聚酰胺膜进行纳滤浓缩。采用中心复合旋转设计(CCRD)22 方法,以提取时间(11.55-138 min)和温度(16.87-53.3°C)为自变量,固定质量与乙醇溶液浓度比(pH1.0 时为 1:25 g/mL)、粒径(1mm)和超声功率(52.8W)进行 UA 提取。获得的最大浓度分别为:花青素 700.94 mg CE/100 g,类黄酮 945.21 mg QE/100 g,酚 133.19 mg GAE/g,抗氧化活性 IC50 为 24.36 μg/mL。鉴定出的主要酚类化合物包括矢车菊素-3-葡萄糖苷、飞燕草素-3-葡萄糖苷和各种酸,如丁香酸和没食子酸。纳滤可成功浓缩这些化合物,将其产率提高 45%。UA 和 NF 可用于可持续提取。

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