Division of Surgery and Interventional Science, University College London, London NW3 2PF, UK.
Department of Renal Medicine, University College London, London NW3 2PF, UK.
Int J Mol Sci. 2024 May 7;25(10):5061. doi: 10.3390/ijms25105061.
Acute kidney injury (AKI) is common following liver transplantation and is associated with liver ischeamia reperfusion (IR) injury. The purpose of this study was to use a mouse model of liver IR injury and AKI to study the role of Neutrophil Gelatinase Associated Lipocalin (NGAL), a biomarker of AKI, in liver IR injury and AKI. We demonstrate an adapted, reproducible model of liver IR injury and AKI in which remote ischemic preconditioning (RIPC) by repeated episodes of hindleg ischemia prior to liver IR reduced the severity of the IR injury. In this model, serum NGAL at 2 h post reperfusion correlated with AKI development early following IR injury. This early rise in serum NGAL was associated with hepatic but not renal upregulation of NGAL mRNA, suggesting NGAL production in the liver but not the kidney in the early phase post liver IR injury.
急性肾损伤（AKI）在肝移植后很常见，与肝缺血再灌注（IR）损伤有关。本研究的目的是使用肝 IR 损伤和 AKI 的小鼠模型来研究中性粒细胞明胶酶相关脂质运载蛋白（NGAL）在肝 IR 损伤和 AKI 中的作用，NGAL 是 AKI 的生物标志物。我们证明了一种改良的、可重复的肝 IR 损伤和 AKI 模型，其中在肝 IR 前通过多次后肢缺血进行远程缺血预处理（RIPC）可减轻 IR 损伤的严重程度。在该模型中，再灌注后 2 小时的血清 NGAL 与 IR 损伤后早期 AKI 的发生相关。血清 NGAL 的早期升高与肝而非肾中 NGAL mRNA 的上调有关，提示肝 IR 损伤后早期肝而非肾中 NGAL 的产生。
