以及：三种经过验证的基因多态性，有助于地中海河水牛实现更可持续的乳制品生产。

, and : Three Validated Gene Polymorphisms Useful for More Sustainable Dairy Production in the Mediterranean River Buffalo.

作者信息

Pauciullo Alfredo, Gaspa Giustino, Zhang Yi, Liu Qingyou, Cosenza Gianfranco

机构信息

Department of Agricultural, Forest and Food Sciences, University of Turin, 10095 Grugliasco, Italy.

College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

出版信息

Animals (Basel). 2024 May 9;14(10):1414. doi: 10.3390/ani14101414.

DOI:10.3390/ani14101414

PMID:38791632

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11117199/

Abstract

The search for DNA polymorphisms useful for the genetic improvement of dairy farm animals has spanned more than 40 years, yielding relevant findings in cattle for milk traits, where the best combination of alleles for dairy processing has been found in casein genes and in . Nowadays, similar results have not yet been reached in river buffaloes, despite the availability of advanced genomic technologies and accurate phenotype records. The aim of the present study was to investigate and validate the effect of four single nucleotide polymorphisms (SNP) in the , , and genes on seven milk traits in a larger buffalo population. These SNPs have previously been reported to be associated with, or affect, dairy traits in smaller populations often belonging to one farm. A total of 800 buffaloes were genotyped. The following traits were individually recorded, monthly, throughout each whole lactation period from 2010 to 2021: daily milk yield (dMY, kg), protein yield (dPY, kg) and fat yield (dFY, kg), fat and protein contents (dFP, % and dPP, %), somatic cell count (SCC, 10 cell/mL) and urea (mg/dL). A total of 15,742 individual milk test day records (2496 lactations) were available for 680 buffalo cows, with 3.6 ± 1.7 parities (from 1 to 13) and an average of 6.1 ± 1.2 test day records per lactation. Three out four SNPs in the , and genes were associated with at least one of analyzed traits. In particular, the (AJ005430:c.578C>T) gave favorable associations with all yield traits (dMY, = 0.022; dPY, = 0.014; dFY, = 0.029) and somatic cell score (SCS, = 0.032). The (HQ677596: c.536C>T) was positively associated with SCS ( = 0.005) and milk urea ( = 0.04). Favorable effects on daily milk yield (dMY, = 0.028), fat (dFP, = 0.027) and protein (dPP, = 0.050) percentages were observed for the . Conversely, the did not show any association with milk traits. This is the first example of a confirmation study carried out in the Mediterranean river buffalo for genes of economic interest in the dairy field, and it represents a very important indication for the preselection of young bulls destined for breeding programs aimed at more sustainable dairy production.

摘要

寻找对奶牛场动物遗传改良有用的DNA多态性已经跨越了40多年，在牛的乳性状方面取得了相关研究成果，其中已发现酪蛋白基因中存在用于乳制品加工的最佳等位基因组合。如今，尽管有先进的基因组技术和准确的表型记录，但在河水牛中尚未取得类似结果。本研究的目的是在更大的水牛群体中研究和验证在、、和基因中的四个单核苷酸多态性（SNP）对七个乳性状的影响。这些SNP先前已报道在通常属于一个农场的较小群体中与乳制品性状相关或对其有影响。总共对800头水牛进行了基因分型。在2010年至202年的每个完整泌乳期内，每月分别记录以下性状：日产奶量（dMY，千克）、蛋白产量（dPY，千克）和脂肪产量（dFY，千克）、脂肪和蛋白含量（dFP，%和dPP，%）、体细胞计数（SCC，10个细胞/毫升）和尿素（毫克/分升）。共有15742条个体牛奶检测日记录（2496次泌乳）可用于680头奶水牛，胎次为3.6±1.7（从1到13），每个泌乳期平均有6.1±1.2次检测日记录。在、和基因中的四个SNP中有三个与至少一个分析性状相关。特别是，（AJ005430:c.578C>T）与所有产量性状（dMY， = 0.022；dPY， = 0.014；dFY， = 0.029）和体细胞评分（SCS， = 0.032）呈有利关联。（HQ677596: c.536C>T）与SCS（ = 0.005）和牛奶尿素（ = 0.04）呈正相关。观察到对日产奶量（dMY， = 0.028）以及脂肪（dFP， = 0.027）和蛋白（dPP， = 0.050）百分比有有利影响。相反，与乳性状未显示出任何关联。这是在地中海河水牛中对乳业经济利益相关基因进行的首次验证研究实例，它为旨在实现更可持续乳制品生产的育种计划中年轻公牛的预选提供了非常重要的指示。