Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland.
LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, Germany.
Viruses. 2024 May 16;16(5):791. doi: 10.3390/v16050791.
Until recently, the diagnosis of feline infectious peritonitis (FIP) in cats usually led to euthanasia, but recent research has revealed that antiviral drugs, including the nucleoside analog GS-441524, have the potential to effectively cure FIP. Alpha-1-acid glycoprotein (AGP) has been suggested as a diagnostic marker for FIP. However, AGP quantification methods are not easily accessible. This study aimed to establish a Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL) assay on the VetBio-1 analyzer to determine the AGP concentrations in feline serum and effusion samples. Linearity was found in serial dilutions between 1:2000 and 1:32,000; the intra-run and inter-run precision was <5% and <15%, respectively; and AGP was stable in serum stored for at least 8 days at room temperature, at 4 °C and at -20 °C. Cats with confirmed FIP had significantly higher serum AGP concentrations (median: 2954 µg/mL (range: 200-5861 µg/mL)) than those with other inflammatory diseases (median: 1734 µg/mL (305-3449 µg/mL)) and clinically healthy cats (median 235 µg/mL (range: 78-616 µg/mL); < 0.0001). The AGP concentrations were significantly higher in the effusions from cats with FIP than in those from diseased cats without FIP ( < 0.0001). The AGP concentrations in the serum of cats with FIP undergoing GS-441524 treatment showed a significant drop within the first seven days of treatment and reached normal levels after ~14 days. In conclusion, the VetBio-1 SPARCL assay offers a precise, fast and cost-effective method to measure the AGP concentrations in serum and effusion samples of feline patients. The monitoring of the AGP concentration throughout FIP treatment provides a valuable marker to evaluate the treatment's effectiveness and identify potential relapses at an early stage.
直到最近，猫传染性腹膜炎（FIP）的诊断通常导致安乐死，但最近的研究表明，抗病毒药物，包括核苷类似物 GS-441524，有可能有效地治愈 FIP。α-1-酸性糖蛋白（AGP）已被提议作为 FIP 的诊断标志物。然而，AGP 定量方法不易获得。本研究旨在 VetBio-1 分析仪上建立空间接近分析物试剂捕获发光（SPARCL）测定法，以确定猫血清和渗出液样本中的 AGP 浓度。在 1:2000 至 1:32000 的系列稀释中发现了线性关系；批内和批间精密度分别<5%和<15%；并且在室温下、4°C 和-20°C 下储存至少 8 天的血清中 AGP 稳定。确诊为 FIP 的猫的血清 AGP 浓度明显高于其他炎症性疾病（中位数：1734 µg/mL（范围：305-3449 µg/mL））和临床健康猫（中位数 235 µg/mL（范围：78-616 µg/mL））（<0.0001）。患有 FIP 的猫的渗出液中的 AGP 浓度明显高于患有非 FIP 炎症性疾病的猫（<0.0001）。接受 GS-441524 治疗的 FIP 猫的血清 AGP 浓度在治疗的前 7 天内明显下降，约 14 天后恢复正常水平。总之，VetBio-1 SPARCL 测定法提供了一种精确、快速且具有成本效益的方法来测量猫患者的血清和渗出液样本中的 AGP 浓度。在整个 FIP 治疗期间监测 AGP 浓度可以提供有价值的标志物，以评估治疗效果并在早期识别潜在的复发。
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