Key Laboratory of High Altitude Hypoxia Environment and Life Health, School of Medicine, Xizang Minzu University, Xianyang 712082, Shaanxi, China.
Key Laboratory of High Altitude Hypoxia Environment and Life Health, School of Medicine, Xizang Minzu University, Xianyang 712082, Shaanxi, China; Department of Healthcare, the Affiliated Hospital of Xizang Minzu University, Xianyang, Shaanxi 712082, China.
Gene. 2024 Nov 15;927:148617. doi: 10.1016/j.gene.2024.148617. Epub 2024 May 23.
This study aimed to investigate the association between single nucleotide polymorphisms (SNPs) in DPF3 and susceptibility to pulmonary tuberculosis (PTB) in the Northwest Chinese Han population.
Genotyping of four DPF3 SNPs (rs10140566, rs75575287, rs202075571, and rs61986330) was performed using Agena MassARRAY from 488 PTB patients and 488 healthy controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using logistic regression. Multifactor dimensionality reduction (MDR) analysis was employed to investigate the effect of SNP-SNP interactions on PTB risk. The GSE54992 dataset was analyzed using R software to ascertain DPF3 expression levels.
Overall analysis revealed that rs202075571 (allele: OR = 1.31, p = 0.015; CC vs. TT: OR = 1.97, p = 0.049; dominant: OR = 1.33, p = 0.032) and rs61986330 (allele: OR = 1.38, p = 0.010; CA vs. CC: OR = 1.35, p = 0.044; dominant: OR = 1.40, p = 0.019) were associated with an increased PTB risk. Stratified analysis showed that rs10140566 was a PTB risk factor in females, those aged ≤40 and non-smokers, and rs202075571 was associated with PTB risk in individuals aged >40 and smokers, and rs61986330 was associated with PTB risk in males, those aged >40 and smokers. The four SNPs model demonstrated significant predictive potential for PTB risk. Furthermore, DPF3 exhibited higher expression in PTB compared to healthy controls.
DPF3 polymorphisms (rs10140566, rs202075571, and rs61986330) are associated with an increased risk of PTB, providing valuable new insights into the mechanism of PTB.
本研究旨在探讨中国西北汉族人群中 DPF3 单核苷酸多态性（SNP）与肺结核（PTB）易感性的关系。
采用 Agena MassARRAY 对 488 例 PTB 患者和 488 例健康对照者的 4 个 DPF3 SNP（rs10140566、rs75575287、rs202075571 和 rs61986330）进行基因分型。采用 logistic 回归计算比值比（OR）和 95%置信区间（CI）。采用多因子降维（MDR）分析探讨 SNP-SNP 相互作用对 PTB 风险的影响。使用 R 软件对 GSE54992 数据集进行分析，以确定 DPF3 的表达水平。
总体分析显示，rs202075571（等位基因：OR=1.31，p=0.015；CC 与 TT：OR=1.97，p=0.049；显性：OR=1.33，p=0.032）和 rs61986330（等位基因：OR=1.38，p=0.010；CA 与 CC：OR=1.35，p=0.044；显性：OR=1.40，p=0.019）与 PTB 风险增加相关。分层分析显示，rs10140566 是女性、年龄≤40 岁和不吸烟者的 PTB 危险因素，rs202075571 与年龄>40 岁和吸烟者的 PTB 风险相关，rs61986330 与男性、年龄>40 岁和吸烟者的 PTB 风险相关。四 SNP 模型对 PTB 风险具有显著的预测潜力。此外，DPF3 在 PTB 中的表达高于健康对照组。
DPF3 多态性（rs10140566、rs202075571 和 rs61986330）与 PTB 风险增加相关，为 PTB 发病机制提供了有价值的新见解。
Zotero 插件