Sancar G B
J Bacteriol. 1985 Feb;161(2):769-71. doi: 10.1128/jb.161.2.769-771.1985.
A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a DNA photolyase.
一段携带酿酒酵母PHR1基因的3.3千碱基对的PvuII片段已被克隆到大肠杆菌表达载体中,并导入缺乏DNA光解酶的大肠杆菌菌株。观察到大肠杆菌phr-1突变得到了互补,这有力地表明酵母PHR1基因编码一种DNA光解酶。