嗜碱芽孢杆菌C-125木聚糖酶基因在大肠杆菌中的分子克隆与表达
Molecular cloning and expression of the xylanase gene of alkalophilic Bacillus sp. strain C-125 in Escherichia coli.
作者信息
Honda H, Kudo T, Horikoshi K
出版信息
J Bacteriol. 1985 Feb;161(2):784-5. doi: 10.1128/jb.161.2.784-785.1985.
Abstract
The gene for xylanase A of alkalophilic Bacillus sp. strain C-125 was cloned in Escherichia coli with pBR322. The plasmid pCX311 contained 2.6- and 2.0-kilobase-pair HindIII fragments. The characteristics of the purified pCX311-encoded xylanase were the same as those of purified xylanase A from alkalophilic Bacillus sp. strain C-125.
摘要
嗜碱芽孢杆菌C-125的木聚糖酶A基因在大肠杆菌中利用pBR322进行克隆。质粒pCX311含有2.6千碱基对和2.0千碱基对的HindIII片段。纯化的pCX311编码的木聚糖酶的特性与嗜碱芽孢杆菌C-125纯化的木聚糖酶A的特性相同。
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