Zingué Stéphane, Tchoupang Edwige Nana, Madji Linda Takou, Pehuie Fomat Boris Hugor, Mafogang Borelle, Njamen Dieudonné, Mendimi Joseph Marie Nkodo
Department of Pharmacotoxicology and Pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaounde 1, Yaounde, Cameroon.
Department of Medical and Biomedical Engineering, Higher Technical Teachers' Training College, University of Ebolowa, Ebolowa, Cameroon.
Front Pharmacol. 2024 May 16;15:1389976. doi: 10.3389/fphar.2024.1389976. eCollection 2024.
Despite efforts, breast cancer remains associated with a high incidence and mortality rate. also known as "Njansang," is a plant used for cancer treatment. While several reports on the anticancer potential of its leaves exist, little is known about its seed oil. This study aimed to evaluate the and anti-breast cancer activity of "Njansang" seed oil. The inhibitory effect of "Njansang" seed oil was determined using MTT and CCK-8 dye reduction assays. Breast cancer was induced with DMBA and promoted with EV (1 mg/kg) for 4 weeks in ovariectomized rats (menopausal condition). Evaluated parameters included tumor incidence, tumor mass and volume, histopathology, breast cancer biomarker CA 15-3, antioxidant status (CAT, GSH, MDA, NO, SOD), TNF-α and INFγ levels, lipid profile (total cholesterol, LDL-cholesterol, triglycerides and HDL-cholesterol), as well as toxicity parameters (ALT, AST, creatinine). "Njansang" oil significantly reduced the growth of ER+ (MCF-7) and triple negative (MDA-MB 231) adenocarcinoma cells as well as tumor incidence, tumor mass and CA 15-3 levels . It exhibited antioxidant activity, characterized by an increase in SOD and catalase activities, GSH levels and decreased MDA levels compared to the DMBA group. TNF-α and INF-γ levels were reduced following oil treatment, while total cholesterol, LDL-cholesterol and triglyceride levels were reduced. The aforementioned findings confirm the protective effects of "Njansang" oil on induced breast cancer in ovariectomized rats.
尽管人们做出了努力，但乳腺癌的发病率和死亡率仍然很高。“Njansang”，也被称为一种用于癌症治疗的植物。虽然已有多篇关于其叶子抗癌潜力的报道，但对其种子油却知之甚少。本研究旨在评估“Njansang”种子油的体外和体内抗乳腺癌活性。使用MTT和CCK-8染料还原试验确定“Njansang”种子油的抑制作用。在去卵巢大鼠（绝经状态）中，用二甲基苯并蒽（DMBA）诱导乳腺癌，并用雌二醇（EV，1mg/kg）促进4周。评估参数包括肿瘤发生率、肿瘤质量和体积、组织病理学、乳腺癌生物标志物CA 15-3、抗氧化状态（CAT、GSH、MDA、NO、SOD）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（INFγ）水平、血脂谱（总胆固醇、低密度脂蛋白胆固醇、甘油三酯和高密度脂蛋白胆固醇）以及毒性参数（谷丙转氨酶、谷草转氨酶、肌酐）。“Njansang”油显著降低了雌激素受体阳性（MCF-7）和三阴性（MDA-MB 231）腺癌细胞的生长，以及肿瘤发生率、肿瘤质量和CA 15-3水平。与DMBA组相比，它表现出抗氧化活性，其特征是超氧化物歧化酶（SOD）和过氧化氢酶活性增加、谷胱甘肽（GSH）水平升高以及丙二醛（MDA）水平降低。油处理后TNF-α和INF-γ水平降低，而总胆固醇、低密度脂蛋白胆固醇和甘油三酯水平降低。上述研究结果证实了“Njansang”油对去卵巢大鼠诱导性乳腺癌的保护作用。
