Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Centre of Excellence in Bionanoscience Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Z Naturforsch C J Biosci. 2024 Jun 3;79(5-6):137-148. doi: 10.1515/znc-2023-0166. Print 2024 May 27.
Antimicrobial resistance (AMR) has emerged as a significant and pressing public health concern, posing serious challenges to effectively preventing and treating persistent diseases. Despite various efforts made in recent years to address this problem, the global trends of AMR continue to escalate without any indication of decline. As AMR is well-known for antibiotics, developing new materials such as metal containing compounds with different mechanisms of action is crucial to effectively address this challenge. Copper, silver, and chitosan in various forms have demonstrated significant biological activities and hold promise for applications in medicine and biotechnology. Exploring the biological properties of these nanoparticles is essential for innovative therapeutic approaches in treating bacterial and fungal infections, cancer, and other diseases. To this end, the present study aimed to synthesize silver@copper oxide (Ag@CuO) nanoparticles and its chitosan nanocomposite (Chi-Ag@CuO) to investigate their antimicrobial efficacy. Various established spectroscopic and microscopic methods were employed for characterization purposes, encompassing scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Subsequently, the antimicrobial activity of the nanoparticles was assessed through MIC (minimum inhibitory concentration), MBC (minimum bactericidal concentration), and well-disk diffusion assays against , , , and . The size of the CuO-NPs, Ag@CuO, and Chi-Ag@CuO NPs was found to be 70-120 nm with a spherical shape and an almost uniform distribution. The nanocomposites were found to possess a minimum inhibitory concentration (MIC) of 5 μg/mL and a minimum bactericidal concentration (MBC) of 250 μg/mL. Moreover, these nanocomposites generated varying clear inhibition zones, with diameters ranging from a minimum of 9 ± 0.5 mm to a maximum of 25 ± 0.5 mm. Consequently, it is evident that the amalgamation of copper-silver-chitosan nanoparticles has exhibited noteworthy antimicrobial properties in the controlled laboratory environment, surpassing the performance of other types of nanoparticles.
抗菌药物耐药性（AMR）已成为一个重大且紧迫的公共卫生问题，对有效预防和治疗持续性疾病构成严重挑战。尽管近年来为解决这一问题做出了各种努力，但 AMR 的全球趋势仍在不断升级，没有任何下降的迹象。由于 AMR 以抗生素为代表，因此开发具有不同作用机制的含金属化合物等新材料对于有效应对这一挑战至关重要。各种形式的铜、银和壳聚糖已显示出显著的生物活性，有望应用于医学和生物技术领域。探索这些纳米颗粒的生物学特性对于创新的治疗方法治疗细菌和真菌感染、癌症等疾病至关重要。为此，本研究旨在合成银@氧化铜（Ag@CuO）纳米颗粒及其壳聚糖纳米复合材料（Chi-Ag@CuO），并研究其抗菌功效。采用各种已建立的光谱和显微镜方法进行特性表征，包括扫描电子显微镜（SEM）、能谱（EDX）、X 射线衍射（XRD）和傅里叶变换红外光谱（FTIR）。随后，通过 MIC（最低抑菌浓度）、MBC（最低杀菌浓度）和微孔板扩散试验评估纳米颗粒对 、 、 、 的抗菌活性。发现 CuO-NPs、Ag@CuO 和 Chi-Ag@CuO NPs 的粒径为 70-120nm，呈球形且分布均匀。纳米复合材料的最低抑菌浓度（MIC）为 5μg/mL，最低杀菌浓度（MBC）为 250μg/mL。此外，这些纳米复合材料产生了不同大小的清晰抑菌圈，直径范围从最小的 9±0.5mm 到最大的 25±0.5mm。因此，显然铜-银-壳聚糖纳米颗粒的结合在受控的实验室环境中表现出了显著的抗菌性能，超过了其他类型纳米颗粒的性能。
