Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan,, 430068, China.
College of Bioengineering and Food, Hubei University of Technology, No. 28 Nanli Road, Wuhan, Hubei Province, China.
World J Microbiol Biotechnol. 2024 Jun 1;40(7):228. doi: 10.1007/s11274-024-04028-5.
Doramectin, an essential animal anthelmintic, is synthesized through the fermentation process of Streptomyces avermitilis. This study delves into the transcriptomic profiles of two strains, namely the doramectin-producing wild-type S. avermitilis N72 and its highly doramectin-producing mutant counterpart, S. avermitilis XY-62. Comparative analysis revealed 860 up-regulated genes and 762 down-regulated genes in the mutant strain, notably impacting the expression of key genes pivotal in doramectin biosynthesis, including aveA1, aveA2, aveA3, aveA4, aveE, and aveBI. These findings shed light on the molecular mechanisms underpinning the heightened doramectin production in S. avermitilis XY-62, presenting promising avenues for optimizing doramectin production processes.
多拉菌素是一种重要的动物驱虫药，通过阿维链霉菌的发酵过程合成。本研究深入探讨了两种菌株的转录组图谱，即生产多拉菌素的野生型阿维链霉菌 N72 及其高产多拉菌素的突变体阿维链霉菌 XY-62。比较分析显示，突变株中有 860 个上调基因和 762 个下调基因，显著影响了多拉菌素生物合成关键基因的表达，包括 aveA1、aveA2、aveA3、aveA4、aveE 和 aveBI。这些发现揭示了阿维链霉菌 XY-62 中多拉菌素高产的分子机制，为优化多拉菌素生产过程提供了有前景的途径。
