College of Animal Science and Technology, Shihezi University, Shihezi, China.
J Anim Physiol Anim Nutr (Berl). 2024 Sep;108(5):1524-1536. doi: 10.1111/jpn.13995. Epub 2024 Jun 2.
This study investigated the effects of cottonseed meal protein hydrolysate (CPH) on the growth performance, carcass characteristics, serum biochemical indices, intestinal morphology, and enzyme activities of yellow-feather broilers. We randomly divided 240 chicks into four groups, each with six replicates: a basal diet with 0% (CON), 1% (LCPH), 3% (MCPH), or 5% (HCPH) CPH. The trail spanned 63 days and included three phases: Days 1-21, 22-42, and 43-63. Increased average daily gain (ADG) and decreased ratio of feed to gain (F/G) with LCPH were observed in 21-day-old broilers (P < 0.05). MCPH led to higher ADG and average daily feed intake (ADFI) in 42-day-old broilers (P < 0.05). Additionally, CPH supplementation resulted in increased dressing percentage, percentage of half-eviscerated yield, percentage of eviscerated yield, breast muscle rate, and leg muscle rate were observed (P < 0.05) with diet. The serum levels of total protein (TP), high-density lipoprotein cholesterol (HDL-C), calcium (Ca), and phosphorus (P) were enhanced, and blood urea nitrogen (BUN) and triglyceride (TG) levels decreased with diet and CPH (P < 0.05). CPH increased the length of the jejunum and ileum and the weight of the duodenum, jejunum, and ileum in 21-day-old broilers (P < 0.05). Alterations in the duodenal villus structure in broilers occurred on Days 21 and 42, and the CPH groups performed better; however, a similar change occurred in the jejunum on Days 42 and 63 (P < 0.05). MCPH and HCPH enhanced trypsin activity in the duodenum of 21-day-old and 63-day-old broilers (p < 0.05). Chymotrypsin activity increased (P > 0.05) in the duodenum of 63-day-old broilers fed MCPH. Lipase activity increased (P < 0.05) in the jejuna of 21-day-old broilers treated with HCPH. CPH increased trypsin activity in the ilea of 21-day-old broilers (P < 0.05). These results showed that CPH influenced the growth performance, carcass characteristics, serum biochemical indices, and intestinal morphology of yellow-feather broilers, which are related to growth stage. The recommended CPH level in broilers is 1% before 21 days of age and 3% after 21 days of age.
本研究旨在探讨棉籽蛋白水解物（CPH）对黄羽肉鸡生长性能、胴体特性、血清生化指标、肠道形态和酶活性的影响。我们将 240 只小鸡随机分为 4 组，每组 6 个重复：基础日粮中分别添加 0%（CON）、1%（LCPH）、3%（MCPH）和 5%（HCPH）CPH。试验期为 63 天，分为三个阶段：第 1-21 天、第 22-42 天和第 43-63 天。结果表明，21 日龄时，添加 LCPH 组肉鸡的平均日增重（ADG）提高，料重比（F/G）降低（P<0.05）。42 日龄时，MCPH 组肉鸡的 ADG 和平均日采食量（ADFI）较高（P<0.05）。此外，饲粮中添加 CPH 可提高肉鸡的屠宰率、半净膛率、全净膛率、胸肌率和腿肌率（P<0.05）。饲粮和 CPH 可提高血清总蛋白（TP）、高密度脂蛋白胆固醇（HDL-C）、钙（Ca）和磷（P）水平，降低血尿素氮（BUN）和甘油三酯（TG）水平（P<0.05）。CPH 还可增加 21 日龄肉鸡空肠和回肠的长度以及十二指肠、空肠和回肠的重量（P<0.05）。21 日龄和 42 日龄时，CPH 组肉鸡的十二指肠绒毛结构发生变化，而 42 日龄和 63 日龄时，CPH 组肉鸡的空肠绒毛结构也发生了类似变化（P<0.05）。21 日龄和 63 日龄时，饲粮中添加 MCPH 和 HCPH 可提高十二指肠中胰蛋白酶的活性（P<0.05）。饲粮中添加 MCPH 可提高 63 日龄肉鸡的十二指肠糜蛋白酶活性（P>0.05）。饲粮中添加 HCPH 可提高 21 日龄肉鸡空肠中的脂肪酶活性（P<0.05）。21 日龄时，CPH 可提高肉鸡回肠中的胰蛋白酶活性（P<0.05）。综上所述，CPH 影响黄羽肉鸡的生长性能、胴体特性、血清生化指标和肠道形态，这与生长阶段有关。21 日龄前肉鸡饲粮中 CPH 的推荐添加水平为 1%，21 日龄后为 3%。
Zotero 插件