Whetton A D, Bazill G W, Dexter T M
J Cell Physiol. 1985 Apr;123(1):73-8. doi: 10.1002/jcp.1041230112.
WEHI-3B myelomonocytic leukaemia cells secrete a haemopoietic cell growth factor (HCGF) which facilitates the proliferation and development of multipotential stem cells and committed progenitor cells. Several cloned, nonleukaemic cell lines (FDC-P cells) are absolutely dependent on HCGF and die in the absence of it. In these cell lines, factor dependence is associated with the ability of HCGF to increase glucose uptake, thereby controlling glycolytic flux and intracellular ATP levels. We have now investigated the effects of HCGF on glucose uptake in WEHI-3B cells. At 20 degrees C 2-deoxyglucose uptake could be stimulated by the addition of HCGF to the extracellular medium. L-glucose uptake was markedly lower than 2-deoxyglucose uptake and did not respond to the addition of HCGF. At 37 degrees C no HCGF stimulation of 2-deoxyglucose uptake was found. However, at this temperature HCGF release from WEHI-3B cells was markedly higher than at 20 degrees C. Our experiments indicate that HCGF stimulates the glucose transport system in both WEHI-3 cells and FDC-P cells. The similarities between the WEHI-3B cell and FDC-P2 cell polypeptide phenotype were investigated using two-dimensional isoelectric focussing/poly-acrylamide gel electrophoresis. This revealed a high degree of correlation between the two cell types in their protein constituents, indicating a close relationship between the normal and leukaemic cells. These similarities between WEHI-3B cells and FDC-P2 cells are considered and their relevance to haemopoiesis and leukaemogenesis is discussed.
WEHI - 3B髓单核细胞白血病细胞分泌一种造血细胞生长因子(HCGF),它能促进多能干细胞和定向祖细胞的增殖与发育。几种克隆的非白血病细胞系(FDC - P细胞)对HCGF绝对依赖,若无该因子则会死亡。在这些细胞系中,因子依赖性与HCGF增加葡萄糖摄取的能力相关,从而控制糖酵解通量和细胞内ATP水平。我们现在研究了HCGF对WEHI - 3B细胞葡萄糖摄取的影响。在20℃时,向细胞外培养基中添加HCGF可刺激2 - 脱氧葡萄糖的摄取。L - 葡萄糖摄取明显低于2 - 脱氧葡萄糖摄取,且对添加HCGF无反应。在37℃时,未发现HCGF对2 - 脱氧葡萄糖摄取有刺激作用。然而,在此温度下,WEHI - 3B细胞释放的HCGF明显高于20℃时。我们的实验表明,HCGF刺激了WEHI - 3细胞和FDC - P细胞中的葡萄糖转运系统。使用二维等电聚焦/聚丙烯酰胺凝胶电泳研究了WEHI - 3B细胞和FDC - P2细胞多肽表型之间的相似性。这揭示了两种细胞类型在蛋白质成分上具有高度相关性,表明正常细胞和白血病细胞之间存在密切关系。讨论了WEHI - 3B细胞和FDC - P2细胞之间的这些相似性及其与造血和白血病发生的相关性。