Department of Microbiology, Faculty of Science, Arak Branch, Islamic Azad University, Arak, Iran.
Quantum Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Cell Mol Biol (Noisy-le-grand). 2024 Jun 5;70(6):48-53. doi: 10.14715/cmb/2024.70.6.8.
The study aimed to determine the effects of probiotic consumption during pregnancy and lactation and post-weaning on acute stress-induced anxiety and gut beneficial microbiota of the female offspring mice.The female offspring mice were divided into several groups: intact, control (only stressed), PBS/dam (dams gavaged with PBS), PRO/dam (dams gavaged with probiotics), PRO/dam+off (both dams and offspring gavaged with probiotics), and PBS/dam+off (both dams and offspring gavaged with PBS)The probiotics chosen are mainly L. rhamnosus, B.breve, and B. longum (108 CFU/ml). Foot shock stress will be applied for one hour on the 43rd day after birth. Behavioral tests were conducted using the open field and elevated plus-maze. Corticosterone was measured by ELISA kit, and intestinal microflora with qPCR.The data showed that PRO/dam+off had more entries into open arms compared to the control group and decreased move distance and time spent in closed arms compared to the control group. However, there was no significant difference between the PRO/dam group and the control group. In the open field test, the control group spent less time in the inner zone compared to the intact group and in PRO/dam+off group. Corticosterone hormone was increased in the control group and was decreased in the PRO/dam+off. Bifidobacteria and Lactobacilli decreased in the control group in comparison to the intact group, and in the PRO/dam+off group increased compared with other groups. Maternal and filial supplementation with a multi-strain probiotic mixture increased levels of beneficial bacteria and reduced stress-induced anxiety in mice.
本研究旨在确定孕期和哺乳期及离乳后摄入益生菌对雌性仔鼠急性应激诱导的焦虑和肠道有益微生物群的影响。将雌性仔鼠分为以下几组：完整组、对照组（仅应激）、PBS/母鼠（灌胃 PBS 的母鼠）、PRO/母鼠（灌胃益生菌的母鼠）、PRO/母鼠+仔鼠（母鼠和仔鼠均灌胃益生菌）和 PBS/母鼠+仔鼠（母鼠和仔鼠均灌胃 PBS）。所选益生菌主要为 L. rhamnosus、B. breve 和 B. longum（108 CFU/ml）。出生后第 43 天进行一小时足部电击应激。采用旷场和高架十字迷宫进行行为测试。通过 ELISA 试剂盒和 qPCR 检测皮质酮和肠道微生物群。结果显示，与对照组相比，PRO/母鼠+仔鼠组进入开放臂的次数更多，而与对照组相比，进入封闭臂的移动距离和时间减少。然而，PRO/母鼠组与对照组之间没有显著差异。在旷场测试中，与完整组相比，对照组在内部区域的停留时间减少，而在 PRO/母鼠+仔鼠组中。与完整组相比，对照组皮质酮激素增加，而 PRO/母鼠+仔鼠组减少。与完整组相比，对照组双歧杆菌和乳酸杆菌减少，而 PRO/母鼠+仔鼠组与其他组相比增加。母鼠和仔鼠补充多菌株益生菌混合物可增加有益菌水平并减轻应激诱导的焦虑。
