Institute of Medicinal Plant Cultivation, School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Kunming, Yunnan, China.
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.
Sci Rep. 2024 Jun 5;14(1):12948. doi: 10.1038/s41598-024-63477-y.
Viral diseases are becoming an important problem in Amorphophallus production due to the propagation of seed corms and their trade across regions. In this study, combined-High-Throughput Sequencing, RT-PCR, electron microscopy, and mechanical inoculation were used to analyze virus-like infected Amorphophallus samples in Yunnan province to investigate the distribution, molecular characterization, and diversity and evolution of Amorphophallus-infecting viruses including three isolates of dasheen mosaic virus and three orthotospoviruses: mulberry vein banding associated virus (MVBaV), tomato zonate spot virus (TZSV) and impatiens necrotic spot virus (INSV). The results showed that DsMV is the dominant virus infecting Amorphophallus, mixed infections with DsMV and MVBaV to Amorphophallus were quite common in Yunnan province, China. This is the first report on infection of Amorphophallus with MVBaV, TZSV, and impatiens necrotic spot virus (INSV) in China. This work will help to develop an effective integrated management strategy to control the spread of Amorphophallus viral diseases.
病毒病在魔芋生产中已成为一个重要问题，因为种球的传播及其在地区间的贸易。在这项研究中，采用高通量测序、RT-PCR、电子显微镜和机械接种的方法，对云南省受类病毒感染的魔芋样品进行了分析，以研究侵染魔芋的病毒的分布、分子特征、多样性和进化，包括三个芋花叶病毒分离物和三种正呼肠孤病毒：桑脉带相关病毒（MVBaV）、番茄环斑病毒（TZSV）和凤仙花坏死斑点病毒（INSV）。结果表明，DsMV 是侵染魔芋的主要病毒，DsMV 与 MVBaV 的混合感染在中国云南相当普遍。这是中国首次报道魔芋感染 MVBaV、TZSV 和凤仙花坏死斑点病毒（INSV）。这项工作将有助于制定有效的综合管理策略来控制魔芋病毒病的传播。
