Deng Binge, Zhang Yuqing, Qiu Guangyu, Li Jin, Lin Linley Li, Ye Jian
Sixth People's Hospital, School of Medicine & School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Hunan Institute of Advanced Sensing and Information Technology, Xiangtan University, Xiangtan, 411105, P. R. China.
Small. 2024 Oct;20(40):e2402235. doi: 10.1002/smll.202402235. Epub 2024 Jun 7.
The field of second near-infrared (NIR-II) surface-enhanced Raman scattering (SERS) nanoprobes has made commendable progress in biomedicine. This article reviews recent advances and future development of NIR-II SERS nanoprobes. It introduces the fundamental principles of SERS nanoprobes and highlights key advances in the NIR-II window, including reduced tissue attenuation, deep penetration, maximized allowable exposure, and improved photostability. The discussion of future directions includes the refinement of nanoprobe substrates, emphasizing the tailoring of optical properties of metallic SERS-active nanoprobes, and exploring non-metallic alternatives. The intricacies of designing Raman reporters for the NIR-II resonance and the potential of these reporters to advance the field are also discussed. The integration of artificial intelligence (AI) into nanoprobe design represents a cutting-edge approach to overcome current challenges. This article also examines the emergence of deep Raman techniques for through-tissue SERS detection, toward NIR-II SERS tomography. It acknowledges instrumental advancements like improved charge-coupled device sensitivity and accelerated imaging speeds. The article concludes by addressing the critical aspects of biosafety, ease of functionalization, compatibility, and the path to clinical translation. With a comprehensive overview of current achievements and future prospects, this review aims to illuminate the path for NIR-II SERS nanoprobes to innovate diagnostic and therapeutic approaches in biomedicine.
第二近红外（NIR-II）表面增强拉曼散射（SERS）纳米探针领域在生物医学方面取得了值得称赞的进展。本文综述了NIR-II SERS纳米探针的最新进展和未来发展。介绍了SERS纳米探针的基本原理，并重点阐述了NIR-II窗口的关键进展，包括减少组织衰减、深层穿透、最大化允许暴露量以及提高光稳定性。对未来方向的讨论包括纳米探针基底的优化，强调金属SERS活性纳米探针光学性质的定制，以及探索非金属替代物。还讨论了为NIR-II共振设计拉曼报告分子的复杂性以及这些报告分子推动该领域发展的潜力。将人工智能（AI）集成到纳米探针设计中是克服当前挑战的前沿方法。本文还探讨了用于穿透组织SERS检测的深度拉曼技术的出现，朝着NIR-II SERS断层扫描发展。认可了诸如提高电荷耦合器件灵敏度和加快成像速度等仪器方面的进步。文章最后论述了生物安全性、功能化便捷性、兼容性以及临床转化途径等关键方面。通过全面概述当前成就和未来前景，本综述旨在为NIR-II SERS纳米探针在生物医学中创新诊断和治疗方法指明道路。
