New -acylated aminoalkanoic acids from tea roots derived biocontrol agent 15020.

Four new -acylated aminoalkanoic acids, namely clonoroseins E-H (-), together with three previously identified analogs, clonoroseins A, B, and D (-), were identified from the endophytic fungus strain 15020 (CR15020), using Feature-based Molecular Networking (FBMN). The elucidation of their chemical structures, including their absolute configurations, was achieved through spectroscopic analysis combined with quantum chemical calculations. Bioinformatics analyses suggested that an iterative type I HR-PKS (CrsE) generates the polyketide side chain of these clonoroseins. Furthermore, a downstream adenylate-forming enzyme of the PKS (CrsD) was suspected to function as an amide synthetase. CrsD potentially facilitates the transformation of the polyketide moiety into an acyl-AMP intermediate, followed by nucleophilic substitution with either β-alanine or γ-aminobutyric acid to produce amide derivatives. These findings significantly expand our understanding of PKS-related products originating from and also underscore the powerful application of FBMN analytical methods in characterization of new compounds.