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基于全转录组扩增的基因表达谱对牛胚胎质量的评估。

Evaluation of bovine embryo quality based on gene expression profiling using whole-transcriptome amplification.

机构信息

Animal Research center, Hokkaido Research Organization, Hokkaido 081-0038, Japan.

Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan.

出版信息

J Reprod Dev. 2024 Aug 7;70(4):259-263. doi: 10.1262/jrd.2024-007. Epub 2024 Jun 8.

DOI:10.1262/jrd.2024-007
PMID:38852998
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11310381/
Abstract

This study aimed to develop a method to evaluate the quality of bovine in vitro fertilized (IVF) embryos based on gene expression profiling via whole-transcriptome amplification. The expression of 11 developmentally important genes in individual bovine in vivo-derived (IVD) and IVF embryos were examined. Gene expression profiling was conducted by classifying the expression level of each gene in individual embryos as low, medium, or high. The IVF group had a higher (P < 0.01) proportion of embryos with low expression of SOX2, NANOG, and FGF4. In addition, a correlation analysis between the expression levels of each gene in individual embryos demonstrated that the relationship between gene expression differed with respect to IVD and IVF embryos. Our results suggest that the expression profiling of developmentally important genes using IVD embryos as normal controls could be a useful indicator for evaluating the quality of bovine IVF embryos.

摘要

本研究旨在通过全转录组扩增，开发一种基于基因表达谱分析评估牛体外受精（IVF）胚胎质量的方法。检测了个体牛体内（IVD）和 IVF 胚胎中 11 种发育重要基因的表达情况。通过将个体胚胎中每个基因的表达水平分类为低、中、高，进行基因表达谱分析。IVF 组中 SOX2、NANOG 和 FGF4 低表达的胚胎比例较高（P<0.01）。此外，对个体胚胎中每个基因表达水平的相关分析表明，基因表达的关系因 IVD 和 IVF 胚胎而异。我们的结果表明，使用 IVD 胚胎作为正常对照，对发育重要基因的表达谱分析可以作为评估牛 IVF 胚胎质量的有用指标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c155/11310381/0c822315f38e/jrd-70-259-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c155/11310381/0c822315f38e/jrd-70-259-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c155/11310381/0c822315f38e/jrd-70-259-g001.jpg

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本文引用的文献

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Reproduction. 2023 Feb 8;165(3):325-333. doi: 10.1530/REP-22-0269. Print 2023 Mar 1.
2
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency.在牛胚胎中的碱基编辑揭示了 SOX2 在调控多能性方面的种特异性作用。
PLoS Genet. 2022 Jul 5;18(7):e1010307. doi: 10.1371/journal.pgen.1010307. eCollection 2022 Jul.
3
Pluripotent Core in Bovine Embryos: A Review.
牛胚胎中的多能核心：综述
Animals (Basel). 2022 Apr 13;12(8):1010. doi: 10.3390/ani12081010.
4
NANOG is required to form the epiblast and maintain pluripotency in the bovine embryo.NANOG 对于牛胚胎中形成上胚层和维持多能性是必需的。
Mol Reprod Dev. 2020 Jan;87(1):152-160. doi: 10.1002/mrd.23304. Epub 2019 Dec 5.
5
Comparison of gene expression and mitochondria number between bovine blastocysts obtained and .所获得的牛囊胚之间基因表达和线粒体数量的比较。 （注：原文中“obtained and.”表述不完整，可能影响对完整意思的理解）
J Reprod Dev. 2020 Feb 14;66(1):35-39. doi: 10.1262/jrd.2019-100. Epub 2019 Nov 20.
6
BOARD INVITED REVIEW: Post-transfer consequences of in vitro-produced embryos in cattle.BOARD 特邀综述：牛体外生产胚胎的移植后后果。
J Anim Sci. 2019 May 30;97(6):2555-2568. doi: 10.1093/jas/skz116.
7
Genome stability of bovine in vivo-conceived cleavage-stage embryos is higher compared to in vitro-produced embryos.与体外受精产生的胚胎相比，体内受精的牛早期胚胎具有更高的基因组稳定性。
Hum Reprod. 2017 Nov 1;32(11):2348-2357. doi: 10.1093/humrep/dex286.
8
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PLoS One. 2014 Sep 30;9(9):e108139. doi: 10.1371/journal.pone.0108139. eCollection 2014.
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RNA-seq analysis of single bovine blastocysts.牛囊胚的 RNA-seq 分析。
BMC Genomics. 2013 May 25;14:350. doi: 10.1186/1471-2164-14-350.