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[温度对离体叶绿体中光诱导pH梯度的影响]

[Effect of temperature on the photo-induced pH gradient in isolated chloroplasts].

作者信息

Kafalieva D N, Busheva M Kh

出版信息

Biofizika. 1979 Jul-Aug;24(4):676-80.

PMID:38857
Abstract

Experimental data are presented on the change of photoinduced proton transport and velocity of electron transport (according to photoreduction of K3Fe(CN)6) in chloroplasts during heating within the temperature range 25-50 degrees C for 3 and 5 min. Preliminary heating of chloroplasts irreverssibly supresses the ability of chloroplasts membrane to bind the protons. The velocity of electron transport decreases with the increase of heating temperature, the value of photoinduced change of pH also decreases. Heating is suggested to affect the electron transport chain. The velocity of electron transport decreases and induces the inhibition of proton transport. Presence of detergents, such as digitonin and triton X-100 in chloroplast suspension in concentrations not higher then 0.02% supresses the process of photoinduced change of pH.

摘要

给出了在25至50摄氏度范围内加热3分钟和5分钟时,叶绿体中光诱导质子运输变化以及电子运输速度(根据铁氰化钾的光还原)的实验数据。叶绿体的预加热不可逆地抑制了叶绿体膜结合质子的能力。电子运输速度随加热温度的升高而降低,光诱导pH变化值也降低。加热被认为会影响电子运输链。电子运输速度降低并导致质子运输受到抑制。在叶绿体悬浮液中存在浓度不高于0.02%的去污剂(如洋地黄皂苷和曲拉通X-100)会抑制pH光诱导变化过程。

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