Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Laboratory of Advanced Theranostic Materials and Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.
Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
Bioconjug Chem. 2024 Jun 19;35(6):758-765. doi: 10.1021/acs.bioconjchem.4c00234. Epub 2024 Jun 10.
Bacterial keratitis, an ocular emergency, is the predominant cause of infectious keratitis. However, diagnostic procedures for it are invasive, time-consuming, and expeditious, thereby limiting effective treatment for the disease in the clinic. It is imperative to develop a timely and convenient method for the noninvasive diagnosis of bacterial keratitis. Fluorescence imaging is a convenient and noninvasive diagnostic method with high sensitivity. In this study, a type of nitroreductase-responsive probe (NTRP), which responds to nitroreductase to generate fluorescence signals, was developed as an activatable fluorescent probe for the imaging diagnosis of bacterial keratitis. Imaging experiments both and demonstrated that the probe exhibited "turn-on" fluorescence signals in response to nitroreductase-secreting bacteria within 10 min. Furthermore, the fluorescence intensity reached its highest at 4 or 6 h and at 30 min when the excitation wavelength was set at 520 nm. Therefore, the NTRP has the potential to serve as a feasible agent for the rapid and noninvasive fluorescence diagnosis of bacterial keratitis.
细菌性角膜炎是一种眼部急症，也是感染性角膜炎的主要病因。然而，其诊断程序具有侵入性、耗时且迅速，从而限制了临床上对该疾病的有效治疗。因此，开发一种及时、便捷的非侵入性细菌性角膜炎诊断方法势在必行。荧光成像是一种方便、非侵入性的诊断方法，具有较高的灵敏度。在本研究中，我们开发了一种硝基还原酶响应型探针（NTRP），该探针可响应硝基还原酶产生荧光信号，作为一种用于细菌性角膜炎成像诊断的激活型荧光探针。成像实验均表明，探针在 10 分钟内对分泌硝基还原酶的细菌表现出“开启”的荧光信号。此外，当激发波长设置为 520nm 时，探针在 4 或 6 小时和 30 分钟时达到最高荧光强度。因此，NTRP 有可能成为一种快速、非侵入性荧光诊断细菌性角膜炎的可行试剂。
J Am Chem Soc. 2015-5-7
Anal Chem. 2016-5-23
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