Wang H, Wang M, Tang F, Wang L, Han R, Jiang F, Zhan X
Department of Medicine, Huaibei Vocational and Technical College, Huaibei, Anhui 235000, China.
Co-first authors.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2024 May 6;36(2):179-183. doi: 10.16250/j.32.1374.2023034.
To investigate the activity of extracts against dust mites, and to isolate and characterize active ingredient of extracts.
The essential oil components were extracted from rhizome powder by rotary evaporation with methanol as solvents, followed by petroleum ether extraction and rotary evaporation. The essential oil was mixed with Tween-80 at a ratio of 1:1 and diluted into concentrations of 1.000 00%, 0.500 00%, 0.250 00%, 0.125 00%, 0.062 50% and 0.031 25%, while diluted Tween-80 served as controls. essential oil at each concentration (200 μL) was transferred evenly to filter papers containing 100 adult mites, with each test repeated in triplicate, and controls were assigned for each concentration. Following treatment at 25 °C and 75% relative humidity for 24 h, the mean corrected mortality of mites was calculated. The essential oil components were separated by silica gel column chromatography, and the essential oil was prepared in the positive column of medium pressure; and then, each component was collected. Silica gel column chromatography was run with the mobile phase that consisted of petroleum ether solution containing 10% ethyl acetate and pure ethyl acetate, detection wavelength of 254 nm, positive silica gel column as the chromatography column, and room temperature as the column temperature. Each component of the purified essential oil was diluted into 1.000 00% for acaricidal tests. The components with less than 100% acaricidal activity were discarded, and the remaining components were diluted into 50% of the previous-round tests for subsequent acaricidal tests. The components with acaricidal activity were subjected to high-performance liquid chromatography, liquid chromatography-mass spectrometry and pulsed-Fourier transform nuclear magnetic resonance spectroscopy. The structure of active monomer compounds was determined by standard spectral library retrieval and literature review.
essential oil at concentrations of 1.000 00%, 0.500 00%, 0.250 00% and 0.125 00% killed all dust mites, and the corrected mortality was all 100%. Exposure to extracts at an effective concentration of 0.062 50% for 24 hours resulted in 94.33% mortality of dust mites. Six components (A to F) were separated using gel column chromatography, and components D and E both showed a 100% acaricidal activity against dust mites at a concentration of 0.50000%. In addition, Component D was identified as isoeugenol methyl ether, and Component E as β-asarinol.
The extract of essential oil has acaricidal activity, and the isoeugenol methyl ether shows a remarkable acaricidal activity against dust mites.
研究[植物名称]提取物对尘螨的活性，并分离鉴定其提取物的活性成分。
以甲醇为溶剂，通过旋转蒸发从[植物名称]根茎粉末中提取挥发油成分，再经石油醚萃取和旋转蒸发。将挥发油与吐温 - 80按1:1比例混合，稀释成1.000 00%、0.500 00%、0.250 00%、0.125 00%、0.062 50%和0.031 25%的浓度，同时将稀释后的吐温 - 80作为对照。将各浓度的[植物名称]挥发油（200 μL）均匀转移至含有100只成年螨的滤纸上，每个试验重复3次，每个浓度设置对照。在25℃和75%相对湿度下处理24小时后，计算螨的平均校正死亡率。通过硅胶柱色谱分离挥发油成分，在中压正相柱上制备挥发油，然后收集各成分。硅胶柱色谱以含10%乙酸乙酯的石油醚溶液和纯乙酸乙酯为流动相，检测波长为254 nm，正相硅胶柱为色谱柱，柱温为室温。将纯化后的[植物名称]挥发油各成分稀释至1.000 00%进行杀螨试验。杀螨活性低于100%的成分被舍弃，其余成分稀释至前一轮试验浓度的50%进行后续杀螨试验。对具有杀螨活性的成分进行高效液相色谱、液相色谱 - 质谱和脉冲傅里叶变换核磁共振光谱分析。通过标准光谱库检索和文献综述确定活性单体化合物的结构。
浓度为1.000 00%、0.500 00%。0.250 00%和0.125 00%的[植物名称]挥发油杀死了所有尘螨，校正死亡率均为100%。在有效浓度0.062 50%下暴露24小时，[植物名称]提取物导致尘螨死亡率为94.33%。使用凝胶柱色谱分离出6种成分（A至F），成分D和E在浓度为0.50000%时对尘螨的杀螨活性均为100%。此外，成分D被鉴定为异丁香酚甲醚，成分E为β - 细辛醚。
[植物名称]挥发油提取物具有杀螨活性，异丁香酚甲醚对尘螨显示出显著的杀螨活性。
Zotero 插件