利用氧瞬变期间的动态气体交换测量来研究非稳态光呼吸。

Using Dynamic Gas Exchange Measurements During Oxygen Transients to Study Nonsteady-State Photorespiration.

机构信息

Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, MI, USA.

Department of Plant Biology, Michigan State University, East Lansing, MI, USA.

出版信息

Methods Mol Biol. 2024;2792:175-184. doi: 10.1007/978-1-0716-3802-6_14.

DOI:10.1007/978-1-0716-3802-6_14

PMID:38861087

Abstract

Leaf-level gas exchange is widely used to investigate the largest carbon fluxes in illuminated leaves, offering a nondestructive way to investigate the impact of photorespiration on plant carbon balance. Modern commercial gas exchange systems allow high temporal resolution measurements under changing environments, aiding the development of nonsteady-state approaches for measuring dynamic photosynthetic responses. Here, we describe a nonsteady-state technique for acquiring the dynamic response of net CO assimilation to changes in photorespiratory fluxes manipulated by O mole fractions. This technique allows for the screening of plant genotypes with variations in their efficiencies of photorespiration under nonsteady-state conditions.

摘要

叶片水平的气体交换被广泛用于研究受光照叶片中最大的碳通量，为研究光呼吸对植物碳平衡的影响提供了一种非破坏性的方法。现代商业气体交换系统允许在不断变化的环境下进行高时间分辨率的测量，有助于开发用于测量动态光合作用响应的非稳态方法。在这里，我们描述了一种非稳态技术，用于获取净 CO2 同化对由 O 分子分数操纵的光呼吸通量变化的动态响应。该技术允许筛选在非稳态条件下具有不同光呼吸效率的植物基因型。