Shainberg A, Cahan R
Cell Biol Int Rep. 1985 Mar;9(3):265-73. doi: 10.1016/0309-1651(85)90043-8.
When trifluoperazine (TFP), a calmodulin antagonist, was given to chick or rat myoblasts in cultures, formation of multinucleated myotubes was inhibited. The inhibition of cell fusion by TFP in rat cultures prevents the normal increase in the amount of acetylcholine receptors (AChR) and creatine kinase (CK), while the levels of these proteins in chick muscle cultures are hardly affected. Another calmodulin antagonist, compound 48/80, inhibits fusion at doses that correspond closely to its antagonistic effects on calmodulin. Thus, our results suggest a possible role for calmodulin in the regulation of myoblast fusion, but not on the appearance of muscle proteins.
当向培养的鸡或大鼠成肌细胞给予钙调蛋白拮抗剂三氟拉嗪（TFP）时，多核肌管的形成受到抑制。TFP对大鼠培养物中细胞融合的抑制作用可阻止乙酰胆碱受体（AChR）和肌酸激酶（CK）量的正常增加，而鸡肌肉培养物中这些蛋白质的水平几乎不受影响。另一种钙调蛋白拮抗剂化合物48/80，在与其对钙调蛋白的拮抗作用密切对应的剂量下抑制融合。因此，我们的结果表明钙调蛋白在成肌细胞融合的调节中可能起作用，但对肌肉蛋白的出现没有影响。
