Tilton Travis J, Martens Kevin, Lucherk Loni W, Word Alyssa B, Holland Ben P, Lawrence Ty E, Tennant Travis C
Beef Carcass Research Center, West Texas A&M University, Canyon, TX 79016, USA.
Life Products Inc., Norfolk, NE 68701, USA.
Transl Anim Sci. 2024 May 29;8:txae086. doi: 10.1093/tas/txae086. eCollection 2024.
The objective of this study was to determine the efficacy of the direct-fed microbial 10-G upon cattle growth performance, liver and lung health, carcass quality, and yield outcomes, as well as prevalence and enumeration of in feces and lymph nodes. Fed beef heifers ( = 1,400; initial shrunk body weight [] 343.3 ± 36.2 kg) were blocked by the day of arrival and randomly allocated to one of two treatments (0 [negative control, CON] or 2 g of a direct-fed microbial [10-G] that provided 1 billion CFUs per animal per day of , , , , and ) with 10 pens per treatment. Recto-anal mucosal fecal samples (; = 477) and subiliac lymph nodes (; = 479) were collected longitudinally at harvest from 23 to 25 heifers per pen. Data were analyzed using mixed models; pen served as the experimental unit; block and harvest date were random effects. No differences were detected in dry matter intake ( = 0.78), final BW ( = 0.64), average daily gain ( = 0.51), gain to feed ( = 0.71), hot carcass weight ( = 0.54), dressed carcass yield ( = 0.52), 12th rib fat depth ( = 0.13), longissimus muscle area ( = 0.62), calculated empty body fat ( = 0.26), or marbling score ( = 0.82). Distributions of liver scores ( ≥ 0.34), quality grades ( ≥ 0.23), and yield grades ( ≥ 0.11) were also not different between treatments. A tendency was detected for more normal lungs ( = 0.08; 10-G = 65.96%, CON = 61.12%) and fewer inflated lungs at harvest for cattle fed 10-G ( = 0.10; 10-G = 0.29%, CON = 1.16%); other lung outcomes did not differ ( ≥ 0.54). prevalence did not differ for RAM samples ( = 0.41; 10-G = 97.74%, CON = 96.82%) or SLN ( = 0.22; 10-G = 17.92%, CON = 13.66%). concentration of RAM samples ( = 0.25; 10-G = 3.87 log CFU/g, CON = 3.32 log CFU/g) or SLN ( = 0.37; 10-G = 1.46 log CFU/g, CON = 1.14 log CFU/g) also did not differ between treatments at harvest. These results do not demonstrate any difference in live animal performance, carcass characteristics, or carriage for heifers fed 10-G.
本研究的目的是确定直接投喂微生物制剂10-G对牛生长性能、肝脏和肺部健康、胴体品质及产量结果的影响，以及粪便和淋巴结中该微生物的流行率和数量。将育肥牛小母牛（n = 1400；初始空腹体重[IBW] 343.3±36.2千克）按到达日期进行分组，并随机分配到两种处理之一（0[阴性对照，CON]或2克直接投喂微生物制剂[10-G]，每头动物每天提供10亿CFU的嗜酸乳杆菌、干酪乳杆菌、植物乳杆菌、双歧杆菌和粪肠球菌），每种处理10个栏。在屠宰时，从每个栏中纵向采集23至25头小母牛的直肠-肛门黏膜粪便样本（n = 477）和髂下淋巴结样本（n = 479）。数据采用混合模型进行分析；栏作为实验单位；分组和屠宰日期为随机效应。在干物质摄入量（P = 0.78）、最终体重（P = 0.64）、平均日增重（P = 0.51）、增重与饲料比（P = 0.71）、热胴体重（P = 0.54）、胴体出肉率（P = 0.52）、第12肋骨处脂肪厚度（P = 0.13）、背最长肌面积（P = 0.62）、计算得出的空体脂肪（P = 0.26）或大理石花纹评分（P = 0.82）方面未检测到差异。处理组之间肝脏评分（P≥0.34）、质量等级（P≥0.23）和产量等级（P≥0.11）的分布也没有差异。对于投喂10-G的牛，在屠宰时发现肺部更正常的趋势（P = 0.08；10-G组为65.96%，CON组为61.12%）以及肺部充气的情况更少（P = 0.10；10-G组为0.29%，CON组为1.16%）；其他肺部结果无差异（P≥0.54）。直肠-肛门黏膜粪便样本（P = 0.41；10-G组为97.74%，CON组为96.82%）或髂下淋巴结（P = 0.22；10-G组为17.92%，CON组为13.66%）中该微生物的流行率没有差异。屠宰时，直肠-肛门黏膜粪便样本（P = 0.25；10-G组为3.87 log CFU/g，CON组为3.32 log CFU/g）或髂下淋巴结（P = 0.37；10-G组为1.46 log CFU/g，CON组为1.14 log CFU/g）中该微生物的浓度在处理组之间也没有差异。这些结果表明，投喂10-G的小母牛在活体动物性能、胴体特征或该微生物携带方面没有任何差异。
