Bidart Gonzalo N, Hyeuk Se, Alter Tobias Benedikt, Yang Lei, Welner Ditte Hededam
The Novo Nordisk Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, Kgs. Lyngby, DK-2800, Denmark.
RWTH Aachen University, Templergraben 55, 52062, Aachen, Germany.
AMB Express. 2024 Jun 12;14(1):70. doi: 10.1186/s13568-024-01727-y.
High throughput screening (HTS) methods of enzyme variants are essential for the development of robust biocatalysts suited for low impact, industrial scale, biobased synthesis of a myriad of compounds. However, for the majority of enzyme classes, current screening methods have limited throughput, or need expensive substrates in combination with sophisticated setups. Here, we present a straightforward, high throughput selection system that couples sucrose synthase activity to growth. Enabling high throughput screening of this enzyme class holds the potential to facilitate the creation of robust variants, which in turn can significantly impact the future of cost effective industrial glycosylation.
酶变体的高通量筛选（HTS）方法对于开发适用于低影响、工业规模、基于生物的多种化合物合成的强大生物催化剂至关重要。然而，对于大多数酶类别，目前的筛选方法通量有限，或者需要昂贵的底物以及复杂的设置。在此，我们提出了一种直接的高通量选择系统，该系统将蔗糖合酶活性与生长相耦合。实现对这类酶的高通量筛选有可能促进强大变体的创建，这反过来又会对具有成本效益的工业糖基化的未来产生重大影响。
