National Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, P. R. China.
Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, Guangzhou, P. R. China.
Pest Manag Sci. 2024 Oct;80(10):5099-5111. doi: 10.1002/ps.8234. Epub 2024 Jun 12.
Pyriproxyfen is an insect growth regulator (IGR) that is effective against various types of insect pests. However, the molecular mechanism underlying pyriproxyfen effects on insect reproduction remains unclear. Thus, in this study, we attempted to uncover the mechanisms underlying the impact of pyriproxyfen on the reproductive system of the model organism Drosophila melanogaster.
A significant decrease in Drosophila reproduction was observed after pyriproxyfen treatment. The juvenile hormone (JH) titer was significantly increased (120.4%) in the ovary samples of pyriproxyfen-treated flies. Likewise, the concentrations of key enzymes and the expression of key genes related to the JH signaling pathway were also increased in the pyriproxyfen-treated group compared with the control group. Furthermore, pyriproxyfen treatment significantly increased (15.6%) the number of germline stem cells (GSCs) and significantly decreased (17%) the number of cystoblasts (CBs). However, no significant differences were observed in the number of somatic cells. We performed RNA interference (RNAi) on five key genes (Met, Tai, gce, ftz-f1, and hairy) related to the JH signaling pathway in germ cells using the germ cell-specific Gal4 driver. Interestingly, RNAi of the selected genes significantly decreased the number of both GSCs and CBs in pyriproxyfen-treated transgenic flies. These results further validate that pyriproxyfen enhances GSC proliferation by up-regulating JH signaling.
Our results indicate that pyriproxyfen significantly decreases reproduction by affecting germ cells in female adult ovaries. The effect of pyriproxyfen on germ cell proliferation and differentiation is mediated by an increase in JH signaling. This study has significant implications for optimizing pest control strategies, developing sustainable agriculture practices, and understanding the mechanism of insecticide action. © 2024 Society of Chemical Industry.
吡丙醚是一种昆虫生长调节剂（IGR），对多种类型的害虫有效。然而，吡丙醚对昆虫生殖影响的分子机制尚不清楚。因此，本研究试图揭示吡丙醚对模式生物黑腹果蝇生殖系统影响的机制。
吡丙醚处理后，果蝇繁殖能力显著下降。吡丙醚处理组果蝇卵巢样本中保幼激素（JH）滴度显著升高（120.4%）。同样，与对照组相比，吡丙醚处理组中与 JH 信号通路相关的关键酶浓度和关键基因的表达也增加。此外，吡丙醚处理组生殖细胞干细胞（GSCs）数量显著增加（15.6%），囊胚细胞（CBs）数量显著减少（17%）。然而，体细胞数量没有明显差异。我们使用生殖细胞特异性 Gal4 驱动子对与 JH 信号通路相关的五个关键基因（Met、Tai、gce、ftz-f1 和 hairy）在生殖细胞中进行 RNA 干扰（RNAi）。有趣的是，所选基因的 RNAi 显著降低了吡丙醚处理转基因果蝇中 GSCs 和 CBs 的数量。这些结果进一步证实，吡丙醚通过上调 JH 信号增强 GSC 增殖。
我们的结果表明，吡丙醚通过影响雌性成虫卵巢中的生殖细胞，显著降低生殖能力。吡丙醚对生殖细胞增殖和分化的影响是通过增加 JH 信号介导的。本研究对优化害虫防治策略、发展可持续农业实践以及理解杀虫剂作用机制具有重要意义。© 2024 化学工业协会。
