State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Chembiochem. 2024 Aug 19;25(16):e202400316. doi: 10.1002/cbic.202400316. Epub 2024 Jul 22.
With the increasing use of polyethylene glycol (PEG) based proteins and drug delivery systems, anti-PEG antibodies have commonly been detected among the population, causing the accelerated blood clearance and hypersensitivity reactions, poses potential risks to the clinical efficacy and safety of PEGylated drugs. Therefore, vigilant monitoring of anti-PEG antibodies is crucial for both research and clinical guidance regarding PEGylated drugs. The enzyme-linked immunosorbent assay (ELISA) is a common method for detecting anti-PEG antibodies. However, diverse coating methods, blocking solutions and washing solutions have been employed across different studies, and unsuitable use of Tween 20 as the surfactant even caused biased results. In this study, we established the optimal substrate coating conditions, and investigated the influence of various surfactants and blocking solutions on the detection accuracy. The findings revealed that incorporating 1 % bovine serum albumin into the serum dilution in the absence of surfactants will result the credible outcomes of anti-PEG antibody detection.
随着聚乙二醇（PEG）基蛋白和药物递送系统的应用日益增多，人群中通常会检测到抗 PEG 抗体，导致血液清除加速和过敏反应，对 PEG 化药物的临床疗效和安全性构成潜在风险。因此，对抗 PEG 抗体进行警惕性监测对于 PEG 化药物的研究和临床指导都至关重要。酶联免疫吸附测定（ELISA）是检测抗 PEG 抗体的常用方法。然而，不同的研究采用了不同的包被方法、封闭溶液和洗涤溶液，甚至不恰当地使用吐温 20 作为表面活性剂也会导致结果出现偏差。在本研究中，我们建立了最佳的底物包被条件，并研究了各种表面活性剂和封闭溶液对检测准确性的影响。研究结果表明，在不使用表面活性剂的情况下，将 1%牛血清白蛋白纳入血清稀释液中，将获得可靠的抗 PEG 抗体检测结果。
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