Bentley A G, Phillips S M, Kaner R J, Theodorides V J, Linette G P, Doughty B L
J Immunol. 1985 Jun;134(6):4163-9.
Previously, we have described an in vitro model of granulomatous hypersensitivity around Schistosoma mansoni eggs in both the murine model of schistosomiasis and in human schistosomiasis. These studies describe a new model of in vitro granuloma formation that complexes soluble egg antigen from S. mansoni eggs, a partially purified protein derivative of Mycobacterium tuberculosis (PPD), or bovine serum albumin to carrier beads. Ultrastructural and morphologic evaluations demonstrate that there are initial macrophage interactions, followed by the recruitment of antigen-specific T cells that interact with and recruit macrophages, lymphocytes, granulocytes, and fibroblasts. Finally, there is a stage of granulomatous organization involving fibroblast proliferation and collagen deposition. The in vitro reactivity, defined by a quantitative granuloma index, correlates with in vivo granulomas around S. mansoni eggs in the livers of infected cell donor animals. In vitro granuloma formation against PPD-coated beads correlated with delayed cutaneous hypersensitivity against PPD, which was judged by footpad swelling. The reactions demonstrate antigenic specificity and were intrinsically modulated in a manner that is analogous to that previously shown with the in vitro egg granuloma model. This model of in vitro granuloma formation promises to be a useful tool for elucidating mechanisms of cellular immunity and regulation.
此前，我们已经描述了在曼氏血吸虫病的小鼠模型和人类血吸虫病中，围绕曼氏血吸虫卵的肉芽肿超敏反应的体外模型。这些研究描述了一种体外肉芽肿形成的新模型，该模型将来自曼氏血吸虫卵的可溶性卵抗原、结核分枝杆菌的部分纯化蛋白衍生物（PPD）或牛血清白蛋白与载体珠结合。超微结构和形态学评估表明，最初有巨噬细胞相互作用，随后募集抗原特异性T细胞，这些T细胞与巨噬细胞、淋巴细胞、粒细胞和成纤维细胞相互作用并募集它们。最后，存在一个肉芽肿组织化阶段，涉及成纤维细胞增殖和胶原蛋白沉积。由定量肉芽肿指数定义的体外反应性与感染细胞供体动物肝脏中围绕曼氏血吸虫卵的体内肉芽肿相关。针对PPD包被珠的体外肉芽肿形成与针对PPD的迟发型皮肤超敏反应相关，后者通过足垫肿胀来判断。这些反应表现出抗原特异性，并且以一种类似于先前在体外卵肉芽肿模型中所显示的方式进行内在调节。这种体外肉芽肿形成模型有望成为阐明细胞免疫和调节机制的有用工具。
