Department of Biotechnology and Food Science, Universidad de Burgos (University of Burgos), Burgos, Spain.
Instituto Agroalimentario de Aragón-IA2. Veterinary School. Universidad de Zaragoza (University of Zaragoza), Zaragoza, Spain.
J Med Food. 2024 Jun;27(6):563-574. doi: 10.1089/jmf.2023.0206. Epub 2024 Jun 13.
Antioxidant-related parameters and anti-inflammatory and antimicrobial activities against were assessed in eight North East Spain poplar propolis samples. Propolis extracts (PEs) were obtained using 70% ethanol (PEE) and methanol (PME). Yield and total phenol compounds were higher in PEE. Phenolic acids were analyzed by a high-performance liquid chromatograph-diode array detector. Caffeic and ferulic acids were quantified in all PEE and PME. All samples contained p-coumaric acid (quantified in 6 PEE and in 3 PME). Ascorbic acid was detected in all propolis, but mainly quantified in PME (≤0.37 mg/g PE). Biological properties were tested on PEE. As for antiradical activities, trolox equivalent antioxidant capacity (TEAC) [against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)], ranged between 578 and 4620 µmol trolox/g, 2,2-diphenyl-1-picrylhydrazyl (DPPH) (against DPPH free radical), between 0.049 and 0.094 mg/mL, antioxidant activity against hydroxyl (OH) radical (AOA), between 0.04 and 11.01 mmol uric acid/g, and oxygen radical absorbance capacity (ORAC) against peroxyl (ROO) radical between 122 and 3282 µmol trolox/g. Results of TEAC, AOA, and ORAC were significantly correlated. IC anti-inflammatory activity ranged from 1.08 to 6.19 mg/mL. Propolis showed higher inhibitory activity against CECT934 and CP101 by agar well diffusion ( < .05) (10.5 and 10.2 mm, respectively) than against CP102 (7.0 mm). Data of this research show that North East Spain propolis may be of interest for pharmaceutical and food industry use.
对来自西班牙东北部的 8 种杨树蜂胶样品进行了抗氧化相关参数、抗炎和抗菌活性评估。使用 70%乙醇（EEP）和甲醇（PME）提取蜂胶提取物（PE）。EEP 的产率和总酚类化合物含量较高。通过高效液相色谱-二极管阵列检测器分析了酚酸。所有 EEP 和 PME 中均定量了咖啡酸和阿魏酸。所有样品均含有 p-香豆酸（在 6 个 EEP 和 3 个 PME 中定量）。所有蜂胶均检测到抗坏血酸，但主要在 PME 中定量（≤0.37mg/g PE）。在 EEP 上测试了生物性能。就自由基清除活性而言，trolox 当量抗氧化能力（TEAC）[针对 2,2'-氮杂双（3-乙基苯并噻唑啉-6-磺酸）]范围为 578 至 4620µmol trolox/g，2,2-二苯基-1-苦基肼基（DPPH）[针对 DPPH 自由基]，在 0.049 至 0.094mg/mL 之间，对羟基（OH）自由基的抗氧化活性（AOA），在 0.04 至 11.01mmol 尿酸/g 之间，以及对过氧自由基（ROO）的氧自由基吸收能力（ORAC）在 122 至 3282µmol trolox/g 之间。TEAC、AOA 和 ORAC 的结果显著相关。抗炎活性的 IC 范围为 1.08 至 6.19mg/mL。通过琼脂孔扩散法，蜂胶对 CECT934 和 CP101 的抑制活性较高（<0.05）（分别为 10.5 和 10.2mm），而对 CP102 的抑制活性较低（7.0mm）。本研究的数据表明，西班牙东北部的蜂胶可能对制药和食品工业有兴趣。
