lncRNA 启动子高甲基化 / 促进食管癌恶性进展。
High methylation of the same promoter of lncRNA / promotes malignant progression of esophageal cancer.
机构信息
Second Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
Department of Thoracic Surgery, Shaoxing People's Hospital, Shaoxing, 312000, China.
出版信息
Epigenomics. 2024;16(10):733-752. doi: 10.1080/17501911.2024.2342229. Epub 2024 Jun 4.
This study aimed to investigate the functions of and in esophageal cancer (EC). Bioinformatics analysis, qRT-PCR and western blot were used to analyze the expression levels. Biological functions were evaluated using cell-counting kit 8, colony formation, Transwell assays and flow cytometry. FISH was used to detect subcellular localization, and methylation-specific PCR determined gene methylation levels. Animal experiments validated the impact on tumor progression. and were highly methylated and downregulated in EC. Overexpression of up-regulated the expression of , thereby inhibiting EC cell viability and metastasis, promoting apoptosis and inhibiting tumor growth. Low expression of / mediated by DNA hypermethylation facilitates the malignant progression of EC.
本研究旨在探讨 和 在食管癌（EC）中的作用。采用生物信息学分析、qRT-PCR 和 Western blot 分析其表达水平。通过细胞计数试剂盒 8、集落形成、Transwell 检测和流式细胞术评估生物学功能。荧光原位杂交（FISH）用于检测亚细胞定位，甲基化特异性 PCR 检测基因甲基化水平。动物实验验证了其对肿瘤进展的影响。在 EC 中， 和 高度甲基化和下调。过表达 上调 的表达，从而抑制 EC 细胞活力和转移，促进细胞凋亡和抑制肿瘤生长。DNA 高甲基化介导的 / 低表达促进 EC 的恶性进展。
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