鸡视网膜24000道尔顿蛋白（视锥蛋白）样免疫反应性在大鼠脑桥下部的定位。

Localization of chick retinal 24,000 dalton protein (visinin)-like immunoreactivity in the rat lower brain stem.

作者信息

Kiyama H, Takami K, Hatakenaka S, Nomura I, Tohyama M, Miki N

出版信息

Neuroscience. 1985 Feb;14(2):547-56. doi: 10.1016/0306-4522(85)90309-4.

Abstract

The distribution of visinin, a 24,000 dalton peptide, in the lower brain stem of the rat was examined by means of an indirect immunofluorescent method. Visinin-immunoreactive structures were found to be unevenly distributed only in the neuronal elements. The following neuronal systems were strongly labeled by the antiserum; the Purkinje cell system, mammillotegmental system, habenulointerpeduncular system, the second layer of the superior colliculus, ventral tegmental area, substantia nigra pars lateralis, area medial to the medial geniculate body, parabrachial area, dorsal and ventral nuclei of the lateral lemniscus, pontine reticular formation just medial to the trigeminal principal nucleus, superior olivary nucleus, solitarii nucleus, external layer of the inferior colliculus and spinal trigeminal nucleus. The densities of the labeled fibers in these areas paralleled those of the labeled cells. In addition, highly dense visinin-immunoreactive fiber plexuses were seen in the zona compacta of the substantia nigra, lateral portion of the interpeduncular nucleus, ventral tegmental nucleus of Gudden and vestibular nucleus.

摘要

采用间接免疫荧光法检测了一种24,000道尔顿肽——视锥蛋白在大鼠脑干下部的分布。发现视锥蛋白免疫反应性结构仅在神经元成分中分布不均。抗血清强烈标记了以下神经元系统：浦肯野细胞系统、乳头体被盖系统、缰核脚间系统、上丘第二层、腹侧被盖区、黑质外侧部、内侧膝状体内侧区域、臂旁区、外侧丘系背核和腹核、三叉神经主核内侧的脑桥网状结构、上橄榄核、孤束核、下丘外层和三叉神经脊束核。这些区域中标记纤维的密度与标记细胞的密度平行。此外，在黑质致密部、脚间核外侧部分、古登腹侧被盖核和前庭核中可见高密度的视锥蛋白免疫反应性纤维丛。