Seyoum Moges Kebede, Fayissa Girma Regassa, Geleta Girma Selale
Department of Chemistry, College of Natural Sciences Salale University Fiche Ethiopia.
Food Sci Nutr. 2024 Mar 4;12(6):4151-4159. doi: 10.1002/fsn3.4075. eCollection 2024 Jun.
Ethiopia is the world's largest producer of teff ( Zucc). This research was intended to determine the levels of mineral nutrients (metals and nonmetals) and nonessential metals in the grains of teff. Following the optimization of the method, the samples were wet-digested using reagents (5-mL HNO and 1-mL HClO) at a temperature of 230°C for two and a half hours and analyzed using inductively coupled plasma-optical emission spectrometry. Under the optimum procedure, the coefficients of determination ( ) ranged between .9980 and .9999. The limits of detection and limits of quantification were in the range of 0.036-14.49 and 0.111-43.93 mg/kg, respectively. The recovery ranged from 80.72% to 107.79%, indicating that the method was accurate. The optimized and validated method was applied to quantify the levels of analytes in the teff samples. The overall mean concentrations of the analytes in the three varieties of teff samples were determined (mg/kg) to be in the order P (3890-4853) > K (3040-3784) > Ca (1906-1959) > Mg (1402-1698) > Fe (128-305) > Mn (64-127.8) > Na (50-136.5) > Zn (19.8-27.3) > B (1.8-21.9) > Cu (4.17-6.9) > Ni (2.6-4.05) > Hg (1.8-4.0) > Pb (0.048-3.7) > Cd (0.012-2.09) > As (0.02-0.24), with a % RSD ranging between 0.017 and 11.1. The results revealed that teff grains are a good source of minerals and contain a significant amount of toxic elements, such as Cd, Hg, Pb, and As.
埃塞俄比亚是世界上最大的画眉草（黍稷）生产国。本研究旨在测定画眉草籽粒中的矿质营养元素（金属和非金属）及非必需金属含量。方法优化后，样品在230℃下用试剂（5 mL硝酸和1 mL高氯酸）进行湿消化两小时半，然后用感应耦合等离子体发射光谱法进行分析。在最佳程序下，测定系数（ ）在0.9980至0.9999之间。检测限和定量限分别在0.036 - 14.49和0.111 - 43.93 mg/kg范围内。回收率在80.72%至107.79%之间，表明该方法准确。将优化并验证后的方法应用于定量分析画眉草样品中的分析物含量。测定了三个品种画眉草样品中分析物的总体平均浓度（mg/kg），顺序为磷（3890 - 4853）＞钾（3040 - 3784）＞钙（1906 - 1959）＞镁（1402 - 1698）＞铁（128 - 305）＞锰（64 - 127.8）＞钠（50 - 136.5）＞锌（19.8 - 27.3）＞硼（1.8 - 21.9）＞铜（4.17 - 6.9）＞镍（2.6 - 4.05）＞汞（1.8 - 4.0）＞铅（0.048 - 3.7）＞镉（0.012 - 2.09）＞砷（0.02 - 0.24），相对标准偏差（%RSD）在0.017至11.1之间。结果表明，画眉草籽粒是矿物质的良好来源，且含有大量有毒元素，如镉、汞、铅和砷。
